贯叶金丝桃散的质量标准研究  

Study on Quality Standard of Hypericum perforatum Powder

作　　者：张超[1] 尚若锋[1] 辛任升 粱剑平[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]甘肃农业大学,甘肃兰州730050

出　　处：《动物医学进展》2014年第10期34-37,共4页Progress In Veterinary Medicine

基　　金："十二五"农村领域国家科技计划项目(2011AA10A214)

摘　　要：建立了贯叶金丝桃散的质量标准。采用薄层层析法和RP-HPLC对散剂中金丝桃素进行了定性鉴定和定量测定。结果显示,采用的薄层色谱法专属性强,且重复性较好。建立的RP-HPLC方法简便、准确、重复性好,当金丝桃素在3.21μg/mL^51.40μg/mL时,建立的RP-HPLC条件峰面积对进样量呈良好的线性关系,标准曲线回归方程为Y=45.983X+10.362,(r=1,n=5),平均回收率为105.73%,RSD为1.52%(n=6)。研究表明,所建立的薄层层析法和RT-HPLC可用于贯叶金丝桃散的质量控制标准。The quantitative identification of hypericin in Hypericum perforatum powder was carried out u-sing TLC and RP-HPLC to establish the quality standard of Hypericum perforatum powder.Results showed that TLC method was specific with good repetition.The established RP-HPLC method was simple, accurate and repeatable with good linearity that hypericin was in the range of 3.21 to 51.40 μg/mL.While the standard curve regression equation was Y=45.983X＋10.362 （r=1,n=5）and the average recovery was 105.73% with RSD=1.52% （n=6）.This study confirmed that the established method can be used as quality control standard of Hypericum perforatum powder.

关 键 词：贯叶金丝桃散 RP-HPLC 薄层层析 质量标准 

分 类 号：S859.799.1[农业科学—临床兽医学]