PVA加门腔分流术对肝硬化大鼠肝脏组织中iNOS水平的影响  

Effect of PVA with portocaval shunt on iNOS in cirrhotic rat liver

作　　者：蔡潮农[1] 方瑞君[2] 李培平[1] 张百萌[1] 关晓东[1] 李坚[1] 

机构地区：[1]中山大学附属第五医院,广东珠海519000 [2]北京师范大学珠海校区门诊部

出　　处：《山东医药》2014年第30期14-16,共3页Shandong Medical Journal

基　　金：珠海市科技局医药卫生重大项目(PB20081002)

摘　　要：目的探讨入肝门静脉完全动脉化(PVA)加门腔分流术(PCS)对肝硬化大鼠肝脏诱导型一氧化氮合成酶(iNOS)水平的影响。方法制作肝硬化大鼠模型100只。随机分为A组40例、B组40例、C组20例。A组行PVA+PCS,B组仅行PCS,C组行门静脉阻断30 min+右肾切除。免疫组化法检测术前及术后1、2、4、8周各组肝组织中的iNOS,采用Image-Pro Plus6.0图像分析软件分析结果。结果三组术后各时点iNOS水平均低于术前(P均<0.05),术后1、2、4周A组iNOS水平高于B、C组(P均<0.01),术后8周三组iNOS水平差异无统计学意义。结论 PVA加PCS可上调肝硬化大鼠肝脏组织中iNOS,但这种变化主要集中在术后4周内。Objective To evaluate the impact of inducible nitric oxide synthase （ iNOS） expression following portal vein arterializations（PVA） associated with portocaval shunt （PCS） in cirrhotic rats liver.Methods A total of 100 rats were involved to make cirrhosis model and randomly equally divided into three groups , which were group A （ adopted PVA＋PCS operation）, group B（PCS operation only）, group C（adopted portal vein block for 30 mins and right nephrectomy oper-ation）.iNOS in liver of each group was measured by method of immunohistochemistryat the moment of pre -operation, post-operative 1 week, 2 weeks,4 weeks and 8 weeks.The results were analysed by software Image-Pro Plus 6.0.Results The values of iNOS decreased obviously over time after surgery in three groups , which were significantly lower than the values of pre-operation （all P〈0.05）.The values of iNOS in group A were significantly higher than the other two groups at the mo-ment of postoperative 1, 2 and 4 weeks（all P〈0.01）.Until the moment of postoperative 8 weeks, there was no significant difference between the group A and the other two groups .Conclusions PVA with PCS technique can raise the iNOS ex-pression of liver tissues in cirrhotic rats within postoperative 4 weeks, but this reactions gradually become less obvious after postoperative 4 weeks.

关 键 词：门静脉动脉化 门腔分流术 肝纤维化 诱导型一氧化氮合成酶 

分 类 号：R657.3[医药卫生—外科学]