不同浓度雄黄对胃腺癌细胞凋亡的影响  

Effects of Different Concentrations of Realgar on the Apoptosis of Gastric Adenocarcinoma Cells

作　　者：胡少明[1] 张瀛[1] 陶秀良[1] 熊丽萍[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030 [2]广州军区武汉总医院病理科,武汉430070

出　　处：《医药导报》2014年第10期1306-1309,共4页Herald of Medicine

摘　　要：目的观察不同浓度雄黄对体外胃腺癌细胞株SGC-7901细胞凋亡的影响。方法采用噻唑蓝(MTT)法检测0,10,20,50,80μg·mL-1浓度雄黄对胃腺癌细胞SGC-7901增殖抑制率的影响;采用膜联蛋白Ⅴ/碘化丙啶双染色法及流式细胞仪检测0,20,50,80μg·mL-1浓度雄黄对SGC-7901细胞凋亡的影响。结果 10,20,50,80μg·mL-1浓度雄黄在24和48 h对SGC-7901细胞株的抑制率分别为6.15%,7.54%,42.31%,63.54%以及32.56%,46.14%,64.51%,87.52%,组间差异有统计学意义(P<0.05)。24和48 h后细胞半数抑制浓度分别约45.23,15.34μg·mL-1。0,20,50,80μg·mL-1浓度雄黄作用24 h后诱导的细胞早期凋亡率依次为(11.10±2.10)%,(15.31±1.30)%,(25.81±2.68)%,(43.62±8.51)%,组间差异有统计学意义(P<0.05);晚期凋亡率依次为(1.84±0.25)%,(4.41±0.09)%,(4.37±0.14)%,(5.00±0.10)%,差异无统计学意义(P>0.05)。结论高纯度雄黄可抑制胃腺癌细胞增殖和诱导细胞早期凋亡。Objective To observe the effect of different concentrations of realgar on the apoptosis of gastric adenocarcinoma cell Line SGC-7901. Methods MTT assay was conducted to detect the growth inhibition ratio of SGC-7901 cells treated by different concentrations of realgar（0,10,20,50 and 80 μg·mL^-1.AnnexinⅤ/Propidium iodide double staining method together with flow cytometry were used to detect the effect of realgar at 0,20,50 and 80 μg·mL^-1n the apoptosis of SGC-7901 cells. Results The growth inhibition ratio of SGC-7901 cells by various concentrations of high-purity realgar（10,20,50 and80 μg·mL^-1 was 6.15%,7.54%,42.31%and 63.54%,respectively,24 h after realgar treatment. At 48 h after the treatment,the growth inhibition ratio rose to 32. 56%,46. 14%,64. 51% and 87. 52%,respectively（P 〈0. 05）. Half inhibitory concentration was 45. 23 μg·mL^-1fter 24 h and 15. 34 μg·mL^-1fter 48 h. After treated by various concentrations of pure realgar（0,20,50 and 80 μg·mL^-1 for 24 h,early cell apoptosis rate was（11.10 ±2.10）%,（15.31 ±1.30）%,（25.81 ±2.68）%and（43.62 ±8. 51）%,respectively,significantly different among the four groups（ P〈 0. 05）. The late cell apoptosis rate in each group was（1.84 ±0.25）%,（4.41 ±0.09）%,（4.37 ±0.14）%and（5.00 ±0.10）%,respectively（P 〉0.05）. Conclusion High purity realgar can inhibit the proliferation and induce early apoptosis of gastric adenocarcinoma SGC-7901 cells.

关 键 词：雄黄 胃腺癌 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]