硒对氧自由基引起大鼠脑线粒体损伤的影响  

Effects of Selenium on Oxygen Free Radical Induced Injury of Rat Cerebral Mitochondria

作　　者：高良才[1] 陈婉蓉[1] 田翠平[1] 傅中滇[1] 阮素云 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]上海市预防医学研究所

出　　处：《中国公共卫生》2002年第8期915-917,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目 (No 39670 637)

摘　　要：目的　探讨硒对氧自由基引起的大鼠脑线粒体损伤的影响。方法　采用Fe2 + -半胱氨酸 (Cys)为氧自由基生成系统 ,在体外模仿脑出血或脑外伤引起的氧自由基损伤模型 ,观测不同浓度 (5 0× 10 -5～ 5 0 μmol/L)亚硒酸钠对Fe2 + -Cys致损伤脑线粒体的肿胀程度、膜流动性、MDA含量以及线粒体形态结构的影响。 结果　低浓度硒 (5 0× 10 -4～ 5 0× 10 -2 μmol/L)可明显防止氧自由基对线粒体的损伤 ,高浓度硒 (0 5～ 5 0 μmol/L)则产生毒性作用 ,二者均至剂量—效应关系。结论　低浓度硒有利于延缓衰老和防治某些神经系统疾病。Objective The effects of selenium on oxygen free radical inducing injury of rat cerebral mitochondria were investigated.Methods The oxygen free radicals (OFRs) injured cerebral mitochondria induced by Fe\+\{2+\}-Cys in vitro were exposed to 5.0×10\+\{-5\}-5 0μmol/L. Na\-2SeO\-3 and the changes of injured mitochondria swelling, membrane fluidity, MDA levels and mitochondria structures were observed.Results Low Se concentration (5.0×10\+\{-4\}-5.0×10\+\{-2\}μmol/L ) can inhabit the injury of rat cerebral mitochondria induced by oxygen free radicals while high Se concentration(0.5-5.0μmol/L)cause toxicity and both are with a relationship of dose-dependent response.Conclusion Low Se concentration is helpful to delay aging and against nervous disease.

关 键 词：硒 氧自由基 大鼠 脑线粒体损伤 透射电镜 脑损伤 

分 类 号：R114[医药卫生—卫生毒理学] R977.9[医药卫生—公共卫生与预防医学]