水稻直立穗突变体 ep7的鉴定及其候选基因分析  

Identification of an Erect Panicle Mutant ep7 in Rice and Analysis of the Candidate Gene

作　　者：朱海涛[1] 柯善文[1] 冯小龙[1] 邹龙海[1] 曾秀瑜 张向前[1] 

机构地区：[1]华南农业大学农学院、广东省植物分子育种重点实验室,广东广州510642

出　　处：《华北农学报》2014年第4期44-48,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30900884);广东省自然科学基金项目(9451064201003804);高等学校博士学科点专项科研基金项目(20094404120011)

摘　　要：在粳稻中花11的组培后代中发现1个突变体ep7,其稻穗直立,且株高、有效穗、粒长和千粒质量等均显著降低;利用该突变体与籼稻华粳籼74构建F2分离群体对突变体进行遗传分析,结果表明,该突变表型由1对核隐性基因控制,并用分子标记将该基因定位于第7染色体长臂上,命名为EP7,其与InDel标记ID5198-2和ID4309-1的遗传距离分别为0.7 cM和0.5 cM;生物信息学和序列多态性分析表明,可能是大片段的DNA插入造成候选基因功能缺失,导致该突变表型的产生。A rice mutant erect panicle 7（ep7）,obtained from tissue culture of a japonica variety Zhonghua 11, showed a dense and erect panicle and significantly reduced plant height ,effective panicle number per plant ,grain length and 1000-grain weight.The genetic analysis of a F2 segregation population derived from the cross between ep7 and an indica variety Huajingxian 74 demonstrated that the mutant phenotype was controlled by a single recessive gene on the long arm of chromosome 7,termed EP7,between two InDel markers ID5198-2 and ID4309-1 with 0.7 cM and 0.5 cM genetic distance,respectively.Morever,bioinformatic analysis and sequence polymorphorism re-vealed that the mutant phenotype might be caused by a large DNA fragment insertion in EP7 gene.

关 键 词：水稻 穗部性状 直立穗突变体 基因定位 

分 类 号：Q78[生物学—分子生物学]