利用基因芯片分析科农9204小麦高产氮高效机制  

Analysis of High Yielding and Nitrogen Use Efficiency Mechanism of Wheat Kn9204 Using Gene Microarray

作　　者：孙丽静[1,2] 张玮[1,3] 纪军[1,3] 李辉[4] 李俊明[1,3] 

机构地区：[1]中国科学院遗传与发育生物学研究所农业资源研究中心,河北石家庄050021 [2]河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北石家庄050035 [3]植物细胞与染色体工程国家重点实验室,北京100101 [4]河北省农林科学院粮油作物研究所河北省作物遗传育种实验室,河北石家庄050035

出　　处：《华北农学报》2014年第4期213-220,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划(“973”计划)项目(2011CB100300);转基因生物新品种培育科技重大专项(2008ZX08002-005);国家现代农业产业技术体系建设专项(CARS-03-03B);河北省现代农业产业技术体系小麦创新团队项目

摘　　要：利用Affymetrix小麦基因芯片技术,比较了高产氮高效小麦品种科农9204和对照京411在不同发育时期和不同组织器官的基因表达谱。结果表明,孕穗期根中,2个品种中差异表达的基因共有2 955个,其中科农9204中表达量高于京411的基因为1 201个,科农9204中表达量低于京411的基因为1 754个。孕穗期旗叶中,2个品种中差异表达的基因共有2 738个,其中科农9204中表达量高于京411的基因为1 323个,科农9204中表达量低于京411的基因为1 415个。花后10 d旗叶中,2个品种中差异表达的基因共有2 294个,其中科农9204中表达量高于京411的基因为839个,科农9204中表达量低于京411的基因为1 455个。在孕穗期根、孕穗期旗叶和花后10 d旗叶中,科农9204中表达量均高于京411的基因为220个,科农9204中表达量均低于京411的基因为303个。为验证基因芯片数据的可靠性和重复性,选择4个差异表达基因进行半定量RT-PCR分析,所有基因结果均与芯片检测结果吻合。根据基因功能注释,孕穗期根、孕穗期旗叶、花后10 d旗叶科农9204和京411品种间差异表达的基因GO分类(按照生物过程分类)中,差异最显著的GO分类均为细胞含氮化合物代谢途径,从基因组水平揭示科农9204和京411细胞含氮化合物代谢途径的差异很可能是导致2个品种间产量和氮效率差异的分子机制。Variations in gene expression profiles in high yielding and nitrogen use efficiency wheat variety Kn 9204 and the control variety J411 in different developmental stages and different tissues were analyzed using Affy-metrix wheat microarray .The results indicated that 2 955 differentially expressed genes were detected between the two varieties in root at booting stage ,including 1 201 and 1 754 of genes were up-and down-regulated comparing Kn9204 to J411 ,respectively .2 738 differentially expressed genes were detected between the two varieties in flag leaf at booting stage,including 1 323 and 1 415 of genes were up-and down-regulated comparing Kn9204 to J411, respectively .Besides 2 294 differentially expressed genes were detected between the two varieties in flag leaf 10 days after anthesis ,including 839 and 1 455 of genes were up-and down-regulated comparing Kn 9204 to J411 ,re-spectively.There were 220 and 303 of genes up-and down-regulated comparing Kn9204 to J411 in all the three groups ,including both root and flag leaf at booting stage , and flag leaf 10 days after anthesis , respectively .Semi quantitative RT-PCR was further used to confirm the results of microarray ,and all the four genes gained the same results by the two methods ,indicating that the microarray results were biologically reproducible .To further charac-terize these differentially expressed genes ,we used Gene Ontology （ GO） for their annotation .The results showed that the most significant GO classification （ According to the biological process classification ） of differentially expressed genes between Kn 9204 and J411 in all the three groups （ Root at booting stage ,flag leaf at booting stage and flag leaf 10 days after anthesis） was cellular nitrogen compound metabolic process ,indicating that the difference of cellular nitrogen compound metabolic process between Kn 9204 and J411 was probably to be the molecular mechanism lead-ing to yielding and nitrogen use efficiency difference between the two varieties .

关 键 词：小麦 基因芯片 差异表达基因 半定量RT-PCR 

分 类 号：S512[农业科学—作物学] S143.2