东莞市不同来源副溶血性弧菌的病原学特征分析  

Pathogenic characteristics of Vibrio parahaemolyticus in Dongguan City

作　　者：李宇聪[1] 杨丽[1] 方昌勇[1] 黄胜中[1] 叶雪仪[1] 袁达康[1] 

机构地区：[1]东莞市疾病预防控制中心,广东东莞523000

出　　处：《华南预防医学》2014年第5期440-444,共5页South China Journal of Preventive Medicine

摘　　要：目的了解东莞市不同来源的副溶血性弧菌的病原学特征,为疾病防控提供实验依据。方法实验菌株来源为2012—2013年分别从东莞市食品监测、食物中毒患者及散发病例中分离的43株副溶血性弧菌。根据GB 4789.7—2013对43株副溶血菌株进行生化鉴定及血清学分析;运用K-B纸片法对菌株进行12种抗生素药敏试验;应用实时荧光PCR扩增法检测菌株的tdh、trh基因;并经限制性内切酶NotⅠ酶切后进行脉冲场凝胶电泳(PFGE)分子分型。结果 43株副溶血菌株中,食物中毒患者、散发病例分离株血清型主要为O3∶K6,占所有病例分离株的83.3%(15/18),25株食品分离株血清型呈多样化分布,无优势血清型;43株副溶血性弧菌对氨苄西林(敏感率为0.0%,0/43)和头孢噻吩(敏感率为20.9%,9/43)敏感率低,对环丙沙星、磺胺复合、亚胺培南完全敏感(敏感率为100.0%,43/43);毒力基因结果显示,病例分离株均只携带tdh基因,食品分离株均不携带tdh、trh基因;PFGE分子分型结果显示,腹泻散发病例、食物中毒患者分离株带型相对集中,食品分离株带型相对分散,相同血清型的菌株同源性较高,不同食物中毒患者分离株PFGE带型一致。结论食品分离株种类繁多且致病性较低,病例分离株同源性较高且致病性较高。Objective To explore pathogenic characteristics of Vibrio parahaemolyticus in Dongguan City, to provide experimental basis for disease control and prevention. Methods According to GB 4789. 7-2013, forty-three strains of Vibrio parahaemolyticus, isolated from food monitoring, patients with food poisoning and sporadic patients in 2012 and 2013, were tested for biochemistry and serological identi- fication. The drug resistance to 12 antibiotics was tested by K-B method, tdh and trh genes of the strains were detected by real-time PCR and digested by restriction endonuclease Not I. Then, molecular typing was performed by the pulsed field gel electrophoresis （PFGE）. Results The main serotype of the strains isola- ted from patients was 03： K6, accounting for 83.3 % of all patients （15/18） , while serotype of 25 strains isolated from food showed diversification, without predominant serotype. The drug resistance of strains had low sensitivity to ampicillin （ sensitive rate, 0.0%, 0/43 ） and keflin （ sensitive rate, 20. 9%, 9/43 ）, while high sensitivity to ciprofloxacin, guanimycin and imipenem （sensitive rate, 100.0%, 43/43）. Strains isolated from patients carried tdh gene, while the strains isolated from food had neither tdh nor trh gcne. The result of PFGE indicated that the bands of strains isolated from patients were relatively concen- trated, while the bands of the strains from food were relatively disperse. Strains of the same serotype were highly homologous and PFGE type was found to be consistent with different patients with food poisonings. Conclusion The strains isolated from food had disperse bands and low pathogenicity and those from pa tients had high homology and pathogenicity.

关 键 词：弧菌 副溶血性 电泳 凝胶 脉冲场 

分 类 号：R378.3[医药卫生—病原生物学] R115[医药卫生—基础医学]