一株耐盐葡萄球菌的分离与鉴定  

Isolation and identification of a halotolerant Staphylococcus strain

作　　者：卫文强[1] 邢伟伟[1] 于运运 朱欣茹 房娜[1] 王风玲[1] 季少平[1] 

机构地区：[1]河南大学医学院,河南开封475004

出　　处：《河南大学学报（医学版）》2014年第3期185-187,共3页Journal of Henan University:Medical Science

基　　金：河南大学博士启动基金(132012106)

摘　　要：目的从盐碱地土壤中分离耐盐耐药细菌。方法采用倍比稀释法从土壤中分离不同性状的细菌,分别对这些细菌进行耐盐性实验,将筛选到耐150g/L NaCl的菌株进行生化实验、药敏实验和16sRNA序列分析。结果16sRNA分析表明从土壤中分离到一株耐盐表皮葡萄球菌,能分解乳糖和蔗糖,可在150g/L NaCl中生长,该菌株对青霉素和苯唑西林耐药。结论该耐盐葡萄球菌株的分离将为耐盐基因的克隆及转基因耐盐菌株的应用奠定基础。Objective To isolate the halotolerant bacterium strain from eastern henan plain.Methods By culturing in different salt concentrations,one strain was isolated.The biochemical test,drug sensitive test,16 sRNA sequence homology analysis were carried out to analyze the characterization of the halotolerant bacterium.Results The halotolerant bacterium was according to the 16 sRNA results.The stain was resistant to penicillin and oxacillin.It could oxidize lactose and sucrose.Conclusion The isolation of the halotolerant bacterium would provide materia for the cloning of halotolerant gene and have an application in the transgenic salt-tolerant bacteria.

关 键 词：葡萄球菌 耐盐 16sRNA 鉴定 

分 类 号：R378.1[医药卫生—病原生物学]