中国鸡群禽呼肠孤病毒抗体的检测与分析  

Antibody detection and analysis of avian reovirus in the chicken flocks in China

作　　者：靳继惠[1] 文楚 邵梦瑜[1] 赵烨[1] 于晓慧[1] 袁希民 张立新[2] 张嘉栋[2] 鹿建山[2] 张洪昌[2] 甘孟侯[1] 赵继勋[1] 吕春梅[2] 何佩[1] 赵丽莉[1] 秦秀慧[1] 才学鹏[3] 张国中[1] 

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]北京康牧兽医药械中心,北京海淀100085 [3]中国动物疫病预防控制中心,北京朝阳100125

出　　处：《中国兽医杂志》2014年第8期3-5,9,共4页Chinese Journal of Veterinary Medicine

基　　金：中国动物疫病预防控制中心《禽呼肠孤病毒病的流行病学调查与预防研究》项目资助

摘　　要：为了解我国鸡群中禽呼肠孤病毒(Avian reovirus,ARV)的抗体水平,本研究利用ELISA方法对2010-2013年来自全国19个省市的鸡血清样品进行了ARV抗体检测。在检测的17 058份鸡血清样品中共检出ARV抗体阳性血清156 80份,阳性率为92.83%,其中免疫鸡群的阳性率为98.50%,非免疫鸡群的阳性率为74.72%。除山东省和广东省外,其他各个省市的样本阳性率均在90%以上。商品肉鸡、肉种鸡、商品蛋鸡和蛋种鸡的ARV抗体阳性率分别为72.66%,98.48%,93.40%和95.53%。部分地方品种鸡群的ARV抗体水平也较高。结果表明,我国鸡群ARV感染情况普遍存在,主要表现为一定比例的免疫鸡群抗体过高、未免疫鸡群存在较高的抗体阳性率。To explore the antibody levels of avian reovirus（ARV）in chicken flocks in China, chicken serum samples from 19 provinces between 2010 and 2013 were detected with a commercial ARV antibody enzyme-linked immunosorbent assay（ELISA）kit.15 680 of 17 058（92.83%）serum samples were positive. The positive rates of vaccinated and non-vaccinated chicken flocks were 98.50% and 74.72% respectively. The positive rates of samples from all provinces were up to 90% except for Shandong and Guangdong province. Seropositivity rates for commercial broilers, broiler breeders, commercial layers and egg breed chickens were 72.66%,98.48%,93.40% and 95.53% respectively. ARV antibody levels of some local varieties were also high. The results indicated that ARV infection was common in the chicken flocks in China,mainly presented as overhigh antibody levels of a certain proportion of vaccinated chicken flocks and high antibody positive rateof non-vaccinated chicken flocks.

关 键 词：禽呼肠孤病毒 鸡 ELISA 抗体 阳性率 感染 

分 类 号：S852.659.4[农业科学—基础兽医学]