猪瘟病毒间接ELISA抗体检测试剂盒的临床应用研究  

Application Study on Indirect ELISA Kit to Detect Antibody against Classical Swine Fever Virus

作　　者：徐璐[1] 范学政[1] 梁智选[2] 陈静 余勇[4] 熊仲良[5] 吴赟竑 宁宜宝[1] 郑然[1] 赵启祖[1] 王琴[1] 

机构地区：[1]中国兽医药品监察所,北京海淀100081 [2]天津市动物疫病预防控制中心,天津北辰300402 [3]山东省动物疫病预防控制中心,山东济南250022 [4]四川省动物疫病预防控制中心,四川成都610041 [5]重庆市动物疫病预防控制中心,重庆渝北401120 [6]浙江省动物疫病预防控制中心,浙江杭州310020

出　　处：《中国兽医杂志》2014年第8期36-38,41,共4页Chinese Journal of Veterinary Medicine

基　　金：自治区科技支疆项目计划-新疆部分种猪场猪瘟净化技术的集成研究与示范(编号201291150);重大动物传染病防治科技创新团队(编号2010R50031)

摘　　要：采用猪瘟病毒间接ELISA抗体检测试剂盒对5省/市的猪血清样本进行检测,并与进口ELISA试剂盒相比较,同时采用世界动物卫生组织(OIE)指定方法-荧光抗体病毒中和试验对检测结果有差异的血清样本进行验证,结果显示,两种试剂盒对田间临床样本的符合率为78.1%;猪瘟病毒间接ELISA抗体检测试剂盒与荧光抗体病毒中和试验的符合率高于进口ELISA试剂盒;综合免疫背景分析,猪瘟病毒间接ELISA抗体试剂盒的敏感性和特异性均高于进口试剂盒,更适合在我国进行大规模推广应用。We used CSF Ab I-ELISA kit to detect pig sera collected from 5 provinces compared with IDEXX CSFV ELISA kit and re-tested parts of sera which showed different results from the two kits with fluorescent antibody virus neutralization test（FVNT）.The results showed that the coincident rate of two kits was 78.1%. The coincident rate of CSF Ab I-ELISA kit with FVNT was higher than IDEXX kit with FVNT. According to the results combined with the immune status, the specificity and sensitivity of CSF Ab I-ELISA kit is higher than IDEXX kit. It is more suitable to spread the use of CSF Ab I-ELISA kit in China.

关 键 词：猪瘟 ELISA 免疫抗体 

分 类 号：S852.651[农业科学—基础兽医学]