异氟烷对发育期大鼠脑室管膜下区神经干细胞的影响  

Effects of isoflurane on the neural stem cell in the subventricular zone of neonatal rats

作　　者：罗萧萧 张雪[2] 赵以林[2] 陈欣[2] 

机构地区：[1]华中科技大学同济医学院第二临床学院,武汉430030 [2] 华中科技大学同济医学院附属同济医院麻醉科

出　　处：《临床外科杂志》2014年第8期606-608,共3页Journal of Clinical Surgery

基　　金：国家自然科学基金（NO.81200880）

摘　　要：目的：探讨异氟烷（Isoflurane）对新生大鼠脑室管膜下区神经干细胞的影响。方法将出生后5 d 的20只新生大鼠随机分为对照组（Control 组）、0．5％异氟烷组（ISO 0．5％组）、0．75％异氟烷组（ISO 0．75％）和1．5％异氟烷组（ISO 1．5％）组。免疫荧光方法标记脑室管膜下区的神经干细胞，观察异氟烷作用后神经干细胞凋亡以及增殖情况的变化。结果0．5％、0．75％和1．5％浓度异氟烷可导致新生大鼠室管膜下区神经干细胞增殖减少（P ＜0．05），而对神经干细胞的凋亡没有影响。结论异氟烷浓度依赖性导致新生大鼠室管膜下区神经干细胞增殖减少，而并不影响神经干细胞的凋亡。Objective To investigate the effects of isoflurane（ISO）on the neural stem cell in the subventricular zone of neonatal rats.Methods Twenty rats on postnatal day 5 were randomly divided in-to 4 groups and exposed to varying ISO concentrations（0%,0.5%,0.75%and 1.5%）respectively.Immu-nofluorescence technique was used to label and detect apoptosis and proliferation of the neural stem cells in the subventricular zone.Results The proliferation of neural stem cells in the subventricular zone was sig-nificantly decreased in rats with 0.5%,0.75% and 1.5% ISO exposure（P 〈0.05）and no effects on ap-optosis were observed between the groups.Conclusion In neonatal rats,isoflurane decreases neural stem cell proliferation of the subventricular zone in a concentration dependent manner and it does not affect ap-optosis.

关 键 词：异氟烷 神经干细胞 增殖 凋亡 新生大鼠 

分 类 号：R614[医药卫生—麻醉学]