539例流产绒毛的荧光原位杂交技术结果分析  

539 cases of abortion villus fl uorescence in situ hybridization results

作　　者：欧阳鲁平 雷亚琴 费冬梅 黄红倩 郑陈光 

机构地区：[1]广西壮族自治区妇产医院/妇幼保健院遗传代谢中心实验室,广西南宁530003

出　　处：《中国优生与遗传杂志》2014年第9期82-83,125,共3页Chinese Journal of Birth Health & Heredity

基　　金：十二五国家科技支撑计划项目两项(2012BAI09B04)

摘　　要：目的探讨荧光原位杂交技术在检测染色体数目异常与自然流产的关系及影响因素。方法应用荧光原位杂交技术对539例早期自然流产绒毛进行染色体数目异常检查及其与患者的年龄、流产次数之间的联系。结果 539例流产绒毛标本中,共检测出133例染色体数目异常,其中常染色体三体最多,共计81例(60.9%);性染色体数目异常22例(16.5%);多倍体30例(22.5%);绒毛染色体数目异常与正常年龄组和高龄组之间的比较,高龄组异常发生率高;而流产次数3次及3次以上的染色体数目异常发生率(29.5%)高于流产次数为2次及2次以下者(20.3%)。结论染色体数目异常是胚胎停育流产的重要因素,其中以常染色体三体为主;胚胎停育与孕妇年龄有关;对于流产次数3次及3次以上的流产患者,建议检查绒毛染色体数目异常很有必要。Objective： Discuss fluorescence in situ hybridization technique in the detection of abnormal chromosome number and the relationship between spontaneous abortion and its influence factors. Methods： Application of fluorescence in situ hybridization technique in 539 cases of early abortion villus chromosome aneuploidy checks and the contact between the patient＇s age, number of abortions. Results： Specimens of 539 cases of abortion villus, detected 133 chromosome aneuploidy, the largest autosomal three body, a total of 81 cases （60.9%） . 22 cases of sex chromosome aneuploidy （16.5%） ; Polyploid 30 cases （22.5%） .Villi chromosome aneuploidy and normal age group and the comparison between age groups, the incidence of abnormal high age group; The number of abortions above 3 times and 3 times the incidence of chromosome number （29.5%） , above the number of abortions for 2 times and 2 times the following （20.3%） .Conclusion： Abnormal chromosome number is an important factor of embryonic TingYu abortion, which mainly autosomal three body; Embryo TingYu related to pregnant women age; For abortion number 3 times and 3 times of abortion patients, suggest that it is necessary to check the villi chromosome aneuploidy.

关 键 词：荧光原位杂交 绒毛 染色体 数目异常 胚胎停育 

分 类 号：R714.21[医药卫生—妇产科学]