HPLC法测定参蛇软膏中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Shenshe Ointments by HPLC

作　　者：缪珊[1] 栗艳[2] 李捷[1] 毕琳琳[1] 张雅[1] 石小鹏[2] 

机构地区：[1]第四军医大学药学系天然药物学教研室,西安710032 [2]第四军医大学西京医院药剂科

出　　处：《中国药师》2014年第9期1576-1578,共3页China Pharmacist

基　　金："重大新药创制"科技重大专项项目(编号:2010ZXJ09105-07B);陕西省社会发展攻关计划(编号:2012K14-05-04)

摘　　要：目的：建立参蛇软膏中盐酸小糪碱的含量测定方法。方法：采用高效液相色谱法测定参蛇软膏中盐酸小糪碱的含量,Kromasil C18色谱柱（250 mm×4.6 mm,5μm）;乙腈-0.1%磷酸（41∶59）（每100 ml加入十二烷基磺酸钠0.02 g）为流动相;流速：1.0 ml·min-1;检测波长：320 nm;柱温：室温;进样量：20μl。结果：盐酸小檗碱与其他组分分离良好,在0.059 2~0.296 0 g·L-1范围内线性关系良好（r=0.999 4）;平均加样回收率为99.80%,RSD=0.24%（n=6）。结论：该方法简单、专属性强、重复性好,可用于参蛇软膏的含量测定。Objective： To establish the determination method for berbefine hydrochlofide in Shenshe ointments. Methods： The quantitative analysis of berberine hydrochloride in Shenshe osintments was carried out by HPLC with a Kromsal C18 chromatographic column （250 mm ×4.6 mm, 5 μm）, the mobile phase was acetonitrile-0.1% phosphoric acid （49：51 ） （adding 0.2g sodium dodecylsulphate into 100 ml solution） with the flow rate of 1.0 ml · min^- 1 and the detection wavelength of 320nm, the temperature of column was room temperature, and the injection volume was 20 I＆l. Results： The linear range was 0. 059 2-0. 296 0 g · L^- 1 with good correlation（r =0.999 4）. The average recovery was 99.80% and RSD was 0.24% （n =6）. Conclusion： The established method is accurate, specific and reproducible, and suitable for the determination of berberine hydrochloride in Shenshe ointments.

关 键 词：参蛇软膏 高效液相色谱法 盐酸小糪碱 含量测定 

分 类 号：R927.2[医药卫生—药学]