Isolation and characterization of cyclic lipopeptides from the marine-derived Bacillus licheniformis  

海洋细菌Bacillus licheniformis中环脂肽类化合物的结构鉴定(英文)

作　　者：高都 田黎[2,3] 柏建[1] 徐岷涓[4] 冯蕾[5] 张庆英[1] 林文翰[1] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室,北京100191 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]青岛科技大学,山东青岛266042 [4]上海交通大学系统生物医学研究院,上海200240 [5]上海交通大学分析测试中心,上海200240

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第8期525-532,共8页中国药学（英文版）

基　　金：Grants from COMRA(Grant No.DY125-15-T-01 and SOA(Grant No.2010319123366025-4);National High Technology Development Project(863 Project,Grant No.2011AA10A202-2);National Key Technologies R&D Program(Grant No.2011BAE06B04)

摘　　要：Chromatographic separation of the marine-derived bacterium Bacillus licheniformis resulted in the isolation of two new cyclic lipopeptides named ai-Cl6 surfactin （1） and ai-Cl4 surfactin （2）, together with iso-Cm5 surfactin and iso-Cl6 surfactin. The structures of the new cyclic lipopeptides were determined through extensive spectroscopic analysis. The sequences of the amino acids in cyclic nucleus were established by the ESI-MS/MS fragmentation, which provided an efficient method to detect lipopeptides from bacterium extracts without senaration从海洋细菌Bacillus licheniformis代谢产物中首次分离得到2个新表面活性肽(1,2),以及2个已知化合物(3,4)。应用现代NMR技术解析了它们的化学结构,并应用化学与色谱相结合方法确定了氨基酸的构型。论文还探讨该类化合物的质谱裂解规律,为环脂肽类化合物的结构解析提供了快速分析方法。

关 键 词：Marine-derived bacterium Bacillus licheniformis Cyclic lipopeptides ai-Cl6 surfactin ai-C14 surfactin UPLC-ESI-MS/MS fragmentation 

分 类 号：R284[医药卫生—中药学]