旋覆代赭汤对反流性食管炎家兔食管下括约肌L型钙通道的作用研究  

Regulation of L-type Calcium Channels by Xuanfu Daizhe Soup in the Lower Esophageal Sphincter Smooth Muscle of Rabbit Reflux Esophagitis Model

作　　者：张俊杰[1] 吴茂申[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中医药大学学报》2014年第8期1007-1011,共5页Journal of Zhejiang Chinese Medical University

摘　　要：[目的]研究旋覆代赭汤对酸和胆汁混合致反流性食管炎家兔食管下括约肌(LES)L型钙通道的调控机制。[方法]制备家兔反流性食管炎模型,用硝苯地平(nifedipine)阻断L型钙通道,比较各组LES环行肌L型钙通道阻断前后张力幅度的差异。[结果]L型钙通道阻断前后各组LES环行肌张力比较:正常组、全方组、甘升组、去苦降组、去升降相因组LES环行肌钙释放相、内流相张力均高于模型组(P<0.01),而苦降组、升降相因组、去甘升组LES环行肌钙释放相与模型组相比无显著性差异(P>0.05),正常组与全方组LES环行肌钙释放相、内流相张力相比无显著性差异(P>0.05)。[结论]酸和胆汁混合致反流性食管炎模型家兔LES环行肌张力下降,与L型钙通道功能障碍有关;旋覆代赭汤可以通过调控L型钙通道提高LES环行肌张力,各拆方组中以甘升组作用显著。[Objective]To research the regulation of L-type calcium channels by Xuanfu Daizhe soup in the lower esophageal sphincter（LES） smooth muscle of rabbit reflux esophagitis model induced by mixed perfusion of hydrochloric acid and bile. [Methods]Established the rabbit model of mixed reflux esophagids, nifedipine was used to block L-type calcium channel.The muscle tension of L-type calcium channel were compared among groups in the experiments. [Results]The calcium releasing and flowing phase of LES in the model group was higher than that in the normal group, the whole recipe group, sweet-scending group, the getting rid of bitter-reducing group and the getting rid of lifting and declining group（P〈0.01）. There was no significant difference between the model group and bitter-reducing group, the lifting and declining group and the getting rid of sweet-ascending group（P〉0.05）. There was no significant difference between the normal group and the whole recipe group in calcium releasing and flowing phase of LES（P〉0.05）. [Conclusion]The decreasing of reflux esophagitis model LES tension was relevant with the L-type calcium charmel dysfunction; Xuanfu Daizhe soup could improve the LES tension by regulating L-type calcium channels. The sweet-ascending group had a remarkable effect among those dismande prescription groups.

关 键 词：反流性食管炎 下食管括约肌 平滑肌张力 钙通道 旋覆代赭汤 拆方研究 

分 类 号：R331[医药卫生—人体生理学]