局灶节段性肾小球硬化患者肾组织microRNAs表达分析  

Renal tissue microRNAs expression in patients with focal segmental glomerulosclerosis

作　　者：张昌明[1] 张婉芬[2] 郑春霞[2] 徐峰[2] 曾彩虹[2] 刘志红[2] 

机构地区：[1]南京大学医学院 [2]南京军区南京总医院国家肾脏疾病临床医学研究中心全军肾脏病研究所,南京210016

出　　处：《肾脏病与透析肾移植杂志》2014年第4期301-306,359,共7页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家重点基础研究发展计划(973计划)(2012CB517603);江苏省自然科学基金(BK2011013;BK2011662)

摘　　要：目的:比较局灶节段性肾小球硬化(FSGS)患者与正常对照肾组织中差异表达的microRNAs(miRNAs),探讨miRNAs在FSGS发病中的作用。方法:选取年龄、性别匹配的FSGS患者6例及正常对照3例,留取肾组织标本,提取总RNA。将6个FSGS及3个正常对照样品分别混合后,采用Taq Man低密度芯片(Taq Man Low Density Array)进行miRNAs表达谱分析,筛选出在FSGS患者肾组织中表达丰富并上调的miRNAs分子;选取14例FSGS患者和15例正常对照,采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)方法对筛选出的miRNAs分子进行验证;采用Pearson相关分析FSGS患者肾组织中miRNAs表达水平与临床病理指标之间的相关性。结果:芯片结果显示正常对照组检测到358个miRNAs,FSGS组检测到427个miRNAs,其中109个miRNAs在FSGS肾组织中表达上调。qRT-PCR结果显示与正常对照相比,FSGS患者肾组织中miRNA-155、miRNA-194和miRNA-215表达显著上调(P<0.05)。相关性分析显示,FSGS患者肾组织miRNA-215表达与肾小球硬化比例、总胆固醇水平正相关。结论:FSGS患者肾组织miRNA-155,miRNA-194和miRNA-215表达上调,它们与FSGS发生和发展之间的联系值得进一步研究阐明。Objective ： To investigate the differences of microRNAs （miRNAs） expression between focal segmental glomerulosclerosis （FSGS） and normal kidney tissues, nethodology：Intrarenal miRNA expression was evaluated using TaqMan Low Density Array with pooled RNA samples from six FSGS patients and three normal controls. A set of differentially expressed miRNAs were found, and then validated by quantitative reverse transcription PCR （qRT-PCR） with individual samples. The relationships between miRNAs levels and clinical characters were analyzed by using Pearson correlation analysis. Results：The TaqMan Low Density Array experiment showed that 109 miRNAs were upregulated in the kidney of FSGS patients. After validation by qRT-PCR in individual samples, the intrarenal miRNA-155, miRNA-194 and miRNA-215 expressions were increased significantly in the patients with FSGS compared with normal controls. In addition, intrarenal miRNA-215 level was correlated significantly with serum total cholesterol levels and the proportions of sclerotic glomeruli in the patients. Conclusion： Intrarenal expression of miRNA-155, miRNA-194 and miRNA-215 in FSGS patients ws increased, which suggested these miRNAs may be involved in the pathogenesis of FSGS.

关 键 词：局灶节段性肾小球硬化 肾组织 MICRORNAS 

分 类 号：R692.3[医药卫生—泌尿科学]