蝉花菌丝对糖尿病肾病大鼠的肾脏保护作用机制研究  

Cordyceps Cicadae Prevents the Development of Diabetic Nephropathy Through Up- regulating the Expression of Tubular SIRT1 in Diabetic Rats

作　　者：刘伟敬[1] 唐阳敏 谢淑华[1] 刘玉宁[2] 潘庆军[1] 吴洪銮 陈孝文[1] 刘华锋[1] 

机构地区：[1]广东医学院附属医院肾病研究所,湛江524001 [2]北京中医药大学东直门医院,北京100700

出　　处：《中国中西医结合肾病杂志》2014年第8期665-668,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广东省自然科学基金资助项目(No.S2012040006276);湛江市科技攻关计划项目(No.2013B01056);广东医学院及广东医学院附属医院博士启动基金资助项目(No.B2012080;BK201201)

摘　　要：目的:观察蝉花菌丝对糖尿病肾病(DN)大鼠肾小管沉默信息调节因子2相关酶1(SIRT1)表达及肾小管损伤的影响,探讨蝉花菌丝延缓DN进展的作用机制。方法:链脲佐菌素(STZ)诱导大鼠高血糖,糖尿病所致肾损伤的大鼠模型(DN)与正常大鼠一起随机分为4组:对照组、蝉花组、DN组、DN+蝉花组,饲养24周。观察DN大鼠肾间质α-SMA、SIRT1表达及纤维化程度,检测血肌酐(Scr)、尿素氮(BUN)、尿白蛋白、24 h尿蛋白、尿白蛋白肌酐比(UACR)、肌酐清除率(Ccr)、肾脏指数。结果:DN大鼠肾小管SIRT1表达显著下降,α-SMA表达明显上调,肾小管纤维化加速,BUN、尿白蛋白、尿白蛋白排泄率、24 h尿蛋白等均显著增加;蝉花菌丝治疗能显著提高DN大鼠肾小管SIRT1表达,延缓肾小管纤维化进程,降低BUN、尿白蛋白、尿蛋白及尿白蛋白肌酐比;蝉花菌丝对非糖尿病大鼠上述指标无显著影响。结论:蝉花菌丝可改善DN肾小管病理损伤、延缓DN进展,从而发挥肾脏保护作用,其机制可能与上调肾小管SIRT1表达有关。Objective：To observe the effect of cordyceps cicadae on tubular SIRT1 expression in diabetic rats,and to ex-plore its possible mechanisms of reno - protection under diabetic condition. Methods：The rats were induced into diabetic nephropathy （DN）by intraperitoneal injection of streptozotocin（ STZ）. They engwere randomly divided into four groups with the normal rats：namely control group,cordyceps cicadae group,DN group,DN ＋ cordyceps cicadae group. At the 24th weeks,the protein expression of SIRT1 and α - SMA and the mRNA level of SIRT1 in tubule were studied. Scr,BUN,Urinary albumin,24 h - hour urine protein, UACR,Ccr and renal index were also investigated. Results：Compared with the normal rats,the protein and mRNA expression of SIRT1 was decreased significantly. And the tubular injury characterized as interstitial expansion（INT% ）and α - SMA expression was enhanced in DN rats. At the same time,an increase in BUN,UAE and UP was observed in DN rats. However,cordyceps cica-dae treatment could significantly attenuated the renal lesion,as well as markedly up - regulated the expression of SIRT1,although it had no effect on non - diabetic rats. Conclusion：Cordyceps cicadae may exert reno - protective effect by up - regulating the expres-sion of SIRT1 in renal tubules under diabetes condition.

关 键 词：蝉花菌丝 糖尿病肾病 SIRT1 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]