氯沙坦钾对糖尿病肾病大鼠肾组织p-JAK2、p-STAT3及VEGF表达的影响  

Effect of Losartan Potassium on p- JAK2,p- STAT3 and VEGF of Renal Tissue in the Diabetic Nephropathy Rats

作　　者：刘明[1] 张晓东[1] 方敬爱[1] 孙艳艳[1] 刘文媛[1] 

机构地区：[1]山西医科大学第一医院肾内科,太原030001

出　　处：《中国中西医结合肾病杂志》2014年第8期669-671,共3页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国际科技合作项目(No.2011081066)

摘　　要：目的:探讨氯沙坦钾对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织p-JAK2、p-STAT3及VEGF表达的影响。方法:健康雄性SD大鼠30只随机分为正常对照组(C组)、DN模型组(DN组)、氯沙坦钾组(L组)。采用单次腹腔内注射链脲佐菌素(streptozotocin,STZ)法建立DN大鼠模型,周期12周。实验12周末检测大鼠血糖、Scr、BUN、24 h尿蛋白定量;光镜及电镜下观察大鼠肾组织病理改变;采用免疫组化方法检测大鼠肾组织p-JAK2、p-STAT3、VEGF表达。结果:实验12周末,模型组大鼠血糖、Scr、BUN、24 h尿蛋白定量均高于对照组(P<0.05),肾组织中p-JAK2、p-STAT3、VEGF表达均高于对照组(P<0.05);氯沙坦钾组大鼠Scr、BUN、24 h尿蛋白定量均低于模型组(P<0.05),肾组织p-JAK2、pSTAT3、VEGF表达均低于模型组(P<0.05)。结论:氯沙坦钾可能部分通过调控p-JAK2、p-STAT3及VEGF的表达而发挥肾脏保护作用。Objective：To investigate the effect of Losartan Potassium on p - JAK2,p - STAT3 and VEGF of renal tissue in the diabetic nephropathy rats. Methods：30 SD male rats were divided randomly into control group（group C）,diabetic nephropathy group（group DN）,Losartan Potassium group（group L）. The rats were being intraperitoneal injected with STZ（1% ,45 mg/ kg）to establish diabetic nephropathy rats models,test period 12 weeks. Blood glucose,Scr,BUN,24 - hour urinary protein were measured at the 12th. The renal pathological changes were detected by light microscope and electron microscopy. The expression of p - JAK2, p - STAT3 and VEGF were detected by immunohistochemistry staining sections. Results：After establishing mode for 12 weeks,com-pared with group C,group DN had significant elevated blood glucose,24 - hour urinary protein,Scr,BUN（P ﹤ 0. 05）,and immuno-histochemistry staining displayed that the expression of p - JAK2,p - STAT3,VEGF remarkably increased（P ﹤ 0. 05）. Compared with group DN,group L had significantly lower 24 urinary protein,Scr,BUN（P ﹤ 0. 05）,the expression of p - JAK2,p - STAT3, VEGF were lower than those in group DN（P ﹤ 0. 05）. Conclusion：Losartan Potassium may in part through regulating the expression of p - JAK2,p - STAT3 and VEGF play a protective role in the kidney of diabetic nephropathy rats.

关 键 词：氯沙坦钾糖尿病肾病p-JAK2 P-STAT3 VEGF 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]