芍药苷对黑素细胞生物活性的影响  

Effect of paeoniflorin on biological activity in normal human malanocytes in vitro

作　　者：徐丽丽[1] 郁博[1] 姚如永[1] 陈宏泉[1] 赵秀峰[1] 倪少萍 

机构地区：[1]青岛大学附属医院,青岛266003

出　　处：《中国麻风皮肤病杂志》2014年第4期195-198,共4页China Journal of Leprosy and Skin Diseases

基　　金：山东省中医药科学技术项目(编号:2011-199);山东省科技发展计划(编号:2011YD18012)

摘　　要：目的：检测芍药苷对体外培养正常人表皮黑素细胞增殖活性、酪氨酸酶活性及黑素合成的影响。方法：建立正常人表皮黑素细胞培养体系，加入不同浓度（5-640μg/mL）芍药苷并作用一定时间后，采用MTT法、体外氧化DOPA反应法、NaOH法等分别测定黑素细胞增殖活性、酪氨酸酶活性及黑素含量的变化。结果：40-160μg/mL芍药苷呈剂量依赖性抑制黑素细胞增殖，与对照组比较差异有统计学意义（ P〈0.05）；5-20μg/mL芍药苷对酪氨酸酶活性呈剂量依赖性促进作用，与对照组比较差异有统计学意义（ P〈0.05）；10-20μg/mL芍药苷对黑素细胞黑素合成起促进作用，与对照组比较差异有统计学意义（ P〈0.05）。结论：芍药苷在较高浓度范围内（40-160μg/mL）对体外培养正常人表皮黑素细胞增殖有剂量依赖性抑制作用；在较低浓度范围内（10-20μg/mL）对其黑素合成有促进作用。Objective：To investigate the effect of paeoniflorin on cell proliferation,tyrosinase activity and melanin synthesis in cultured normal human melanocutes. Methods：After paeoniforin （5-640 μg/mL） was added into cultured normal human malanocytes,the effect of paeoniflorin on the proferation of melanocytes,ty-rosinase activity and synthesis of melanin in melanocytes were measured by MTT method,dopamine oxidation method and NaOH method,respectively. Results：40-160 μg/mL paeoniflorin inhibited the growth of mela-nocytes in a concentration-dependent manner （ P〈0.05） . When the concentrations of 5-20μg/mL paeonifor-in were added,the tyrosinase activity was significantly increased in a concentration-dependent manner （ P〈0.05）. When the concentrations of 10 -20μg/mL paeoniforin were added,the melanin synthesis were signifi-cantly increased （ P〈0.05） . The differences in the results mentioned above among the test groups and control group were significant （all Ps〈0.05）.Conclusion： Paeoniflorin in concentrations of 40-160 μg/mL can in-hibit the proliferation in cultured normal human malanocytes in a concentration-dependent manner. When the concentration of 10-20 μg/mL is used the melanin synthesis is promoted.

关 键 词：芍药苷 黑素细胞 酪氨酸酶 

分 类 号：R285[医药卫生—中药学]