黄连解毒汤有效部位对脑缺血大鼠神经元MAP-2、PGP9.5表达及星形胶质细胞活化的影响  

Active Fractions of Huanglian Jiedu Decoction on the MAP- 2 /PGP9. 5Expression and the Impact of Astrocyte Activation after Cerebral Ischemia Rats

作　　者：龙建飞[1] 张秋霞[1] 张建[1] 王雅丽[1] 赵晖[1] 王蕾[1] 边宝林[2] 赵海誉[2] 

机构地区：[1]首都医科大学中医药学院中医络病研究北京市重点实验室,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中医药信息》2014年第5期49-53,共5页Information on Traditional Chinese Medicine

基　　金：国家科技支撑计划课题(No.2008BAI51B02);北京市自然科学基金项目(No.7102014;7122018);北京市属高等学校高层次人才引进与培养计划-长城学者项目资助(No.CIT&TCD20140329);北京市属高等学校人才强教深化计划"中青年骨干人材培养计划"项目(No.PXM2011014226)

摘　　要：目的:观察黄连解毒汤有效部位(总生物碱、总黄酮、总环烯醚萜)对局灶性脑缺血大鼠神经元MAP-2、PGP9.5表达及星形胶质细胞活化的影响,探讨其对神经元-胶质细胞网络的调控作用。方法:中动脉栓塞法建立局灶性脑缺血大鼠模型,SPF级雄性SD大鼠随机分为假手术组、模型组、黄连解毒汤水提物组(800mg/kg)、总生物碱组(44mg/kg)、总黄酮组(50mg/kg)、总环烯醚萜组(80mg/kg),每天灌胃给药1次,连续给药7天。免疫荧光染色结合图像分析技术检测脑缺血大鼠梗死灶周围皮层MAP-2、PGP9.5及星形胶质细胞活化标志蛋白GFAP的表达。结果:脑缺血7天大鼠PGP9.5表达下调,GFAP表达增强,差异较假手术组明显(P<0.01);与模型组相比,黄连解毒汤水提取物组大鼠梗死灶周围神经元MAP-2表达明显上调,GFAP表达下调(P<0.01或P<0.05);总生物碱、总黄酮、总环烯醚萜均可明显上调缺血大鼠梗死灶周围皮层MAP-2、PGP9.5表达,较模型组有统计学差异(P<0.01或P<0.05)。总生物碱还可明显下调GFAP表达,较模型组具有显著性差异(P<0.01)。结论:黄连解毒汤有效部位总生物碱可减轻神经元轴突损伤,促进神经元树突重塑,抑制星形胶质细胞异常活化。Objective ： To investigate the effect of Huanglian Jiedu decoction （HLJDD） which consists of total al- kaloids, total flavonoids and total iridoids the expression of MAP - 2 and PGP9.5 and the impact of astrocyte activation. Probe the regulatory role of neuron - glial cells network. Methods ： Rat models of focal cerebral is- chemia were established. The expressions of MAP - 2, PGP9.5 and GFAP in isehemic penumbra cortex wereobserved by immunofluorescence staining and then analyzed by imaged imagine analysis system. Male Sprague -Dawley rats were randomly divided into sham group, model group, aqueous extract group, total al- kaloids group, total flavonoids group and total iridoids group. The extracts were intragastrically administrated to rats in doses of aqueous extract group （800mg/kg/day）, total alkaloids group （44mg/kg/day）, total fla- vonoids group （50mg/kg/day）, total iridoids group （80mg/kg/day）. Administer each treatment group once every 12h and then sacrifice the rats after seven - day continuous treatment. Results： The expressions of PGP9.5 were excessively decreased, and the expressions of GFAP were increased compared with those of the sham group（P 〈0.01 ）, while aqueous extract could increase the MAP- 2 expression and decrease the GFAP expression compared with those of the model group（ P 〈0.01 or P 〈0.05 ）. Total alkaloids, total flavonoids, total iridoids could increase the MAP - 2 and PGP9.5 expressions compared with those of the model group （P 〈 0.01 or P 〈 0.05 ）. Total alkaloids could excessively decrease the expression of GFAP compared with those of the model group（P 〈0.01 ）. Conclusion： Total alkaloids are inherently capable of reducing the neu- ronal axonal injury, promoting the neuronal dendritic remodeling and inhibiting the abnormal activation of as- trocytes.

关 键 词：黄连解毒汤 脑缺血 MAP-2 PGP9.5 GFAP 

分 类 号：R285.5[医药卫生—中药学]