江浙蝮蛇毒C_4组分的中枢镇痛作用及其机理研究  

Study on Central Analgesic Effect of C_4 Protein Isolated from Snake Venom of Agkistrodon Halys Pallas and Its Mechanism

作　　者：金铭[1] 沈淼山 周荣[1] 阮叶萍[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中国中医药科技》2014年第5期510-512,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家自然科学基金青年科学基金项目(No.81102842)

摘　　要：目的:研究江浙蝮蛇毒镇痛C4组分的中枢镇痛作用并初步探讨其镇痛机理。方法:通过侧脑室给药、脊髓化鼠镇痛实验和足跖定压刺激试验研究C4组分的镇痛作用部位;通过C4组分与阿托品、利血平、纳络酮药物的相互作用,研究其与乙酰胆碱受体、阿片受体间的作用关系。结果:C4组分的镇痛部位主要在脑中枢,且C4组分不通过乙酰胆碱受体作用,而与阿片受体相关。结论:江浙蝮蛇毒C4组分镇痛通过中枢起效,提示江浙蝮蛇毒C4组分可能与增加内源性阿片肽系统的活动有关。Objective：To research the central analgesic effect of C 4 protein in snake venom of Agkistrodon halys Pallas and explore the mechanism.Methods：Using the experiments of spinal cord break , lateral ventricle injection and toe press to research the central analgesic action; the relationships of C4 protein with acetycholine receptors, vesicle transmitters and opiate receptors were explored through the interaction of C 4 prtoein with atro-pine, reserpine and naloxon.Results：C4 protein played a role in CNS and the action of C 4 protein was related with opiate receptors instead of acetycholine receptors.Conclusion：Analgesic effect of C 4 protein was acted on central system, which was related with the endogenous opioid peptide system.

关 键 词：江浙蝮蛇毒 镇痛C4组分 中枢镇痛 阿片肽 乙酰胆碱受体 

分 类 号：R285.5[医药卫生—中药学]