朝鲜白头翁种子后熟调控和育苗关键技术研究  

Study on Seeds After-ripening Regulation and Key Techniques for Seedling of Pulsatilla cernua

作　　者：顾地周[1] 张力凡[1] 王秋爽[1] 张学士[1] 

机构地区：[1]通化师范学院生命科学学院,吉林通化134002

出　　处：《中药材》2014年第7期1126-1129,共4页Journal of Chinese Medicinal Materials

基　　金：吉林省科技发展计划资助项目(20140204002NY)

摘　　要：目的：以朝鲜白头翁种子为供试材料,筛选并确定调控朝鲜白头翁种胚快速发育和种子萌发的主要因素及水平。方法：通过多因子交叉重复试验对影响朝鲜白头翁种胚发育和种子萌发的主要因素及其水平进行探讨。结果：朝鲜白头翁种胚发育成熟和种子萌发的方法为：种子用2.40 mg/L KT、2.80 mg/L GA3和0.30~0.70mg/L 2,4-D混合溶液浸泡24 h,将种子与河砂（1∶2）混合,进行63~70 d的变温处理,变温幅度和时间：白天温度为（23±2）℃,处理14 h,夜间温度为（10±2）℃,处理10 h,子叶胚发生率95.1%,种子发芽率92.3%。结论：解决了朝鲜白头翁种胚发育调控和育苗技术,该技术适合于朝鲜白头翁种苗的工厂化生产。Objective： The seeds of Pulsatilla cernua were used as tested materials for screening and establishing the main factors with different levels to control fast development of seed embryonic and seedlings of Pulsatilla cernua.Methods： The main factors with different levels for development of seed embryonic and seedlings of Pulsatilla cernua were investigated through repeated experiments with multifactorial and cross.Results： The method for development of seedlings and seeds germination of Pulsatilla cernua were to soak the seeds in the mixed solutions with 2.40 mg /L KT,2.80 mg /L GA3 and 0.30 ~ 0.70 mg /L 2,4-D for 24 h.The seeds and sand（ 1∶ 2）were mixed,treated with temperature change in 63 ~ 70 d.The extent of temperature change and time were（ 23 ± 2） ℃ and 14 h in day,while（ 10 ± 2） ℃ and 10 h in night.The incidence rate of the embryo with cotyledons was 95.1%,and the germination rate of seed was 92.3%.Conclusion： The plant regeneration control technology for development of seed embryonic and seedling of Pulsatilla cernua have been solved,which is suitable for industrial seedlings of Pulsatilla cernua.

关 键 词：朝鲜白头翁 种胚 调控 育苗 关键技术 

分 类 号：R282.2[医药卫生—中药学]