罗布麻叶黄酮类成分的UPLC-Q-TOF-MS分析  

Analytical Studies on Flavonoids Constituents in Apocynum venetom Leaves by UPLC-Q-TOF-MS

作　　者：宋建平[1] 许虎[2] 陈菲[2] 刘训红[2] 张奉苏[2] 侯娅[2] 马阳[2] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224005 [2]南京中医药大学,江苏南京210023

出　　处：《中药材》2014年第7期1199-1204,共6页Journal of Chinese Medicinal Materials

基　　金：盐城市2008年科技发展计划(社会发展)项目(YK2008081);江苏省中医药管理局资助项目期(LZ09020)

摘　　要：目的：建立超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对罗布麻叶中黄酮类成分分析的方法。方法：采用Alltima C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-1%醋酸为流动相,梯度洗脱,流速为0.8mL/min,检测波长为254 nm,柱温为30℃。在电喷雾负离子模式下监测采集数据。结果：初步鉴定出11个黄酮类化合物,并同时测定其中6个黄酮的含量。6个黄酮的浓度与峰面积的线性关系良好（r〉0.9996）,加样回收率为94.3%~103.3%。结论：该方法准确可靠,重现性好,可作为罗布麻叶内在质量评价的依据。Objective： To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric（ UPLC-Q-TOF-MS） method for analyzing the flavonoids constituents in Apocynum venetom leaves.Methods： The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL /min.The detection wavelength was at 254 nm and the column temperature was at 30 ℃.Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS.Results： 11 flavonoids constituents being separated and identified,six of them were detected simultaneously.The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area（ r 0.9996）,and the average recoveries of the method were between 94.3% ~103.3%.Conclusion： The method is accurate,reproducible,and can be used for the quality control of Apocynum venetom leaves.

关 键 词：超高效液相色谱-四极杆飞行时间质谱 罗布麻叶 黄酮类 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]