Nesfatin-1对卡巴胆碱诱导的大鼠胃粘膜细胞胃酸分泌的影响及其机制研究  

Effects and Mechanisms of Nesfatin-1 on Carbachol-induced Gastric Acid Secrection in Isolated Rat Gastric Mucosal Cells

作　　者：谢碧云[1] 李学良[1] 周小平[1] 黄琳[1] 林琳[1] 

机构地区：[1]南京医科大学第一附属医院,江苏南京210029

出　　处：《现代生物医学进展》2014年第32期6246-6249,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81070308/H0308)

摘　　要：目的:探讨Nesfatin-1对卡巴胆碱诱导的离体大鼠胃粘膜细胞胃酸分泌的影响及其机制。方法:采用酶解法分离原代SD大鼠胃粘膜细胞。Nesfatin-1(10-1μmol/L)作用大鼠胃粘膜细胞不同时间以及不同浓度Nesfatin-1(10-1、10-2、10-3、10-4μmol/L)作用大鼠胃粘膜细胞0.5 h后,通过14C-氨基比林(14C-Aminopyrine,14C-AP)法检测其对卡巴胆碱(100μmol/L)诱导的大鼠胃粘膜细胞胃酸分泌的影响。将Nesfatin-1(10-1μmol/L)与卡巴胆碱(100μmol/L)共孵育大鼠胃粘膜细胞0.5 h后,通过透射电镜观察胃壁细胞超微结构的变化。结果:Nesfatin-1(10-1μmol/L)作用于大鼠胃粘膜细胞0.5 h、1.0 h及10-1、10-2、10-3μmol/L Nesfatin-1作用于大鼠胃粘膜细胞0.5 h均可明显降低卡巴胆碱诱导的14C-AP摄取量,与卡巴胆碱组相比,差异有统计学意义(P<0.05);Nesfatin-1可影响卡巴胆碱诱导的大鼠胃壁细胞的超微结构,抑制其从静息态向分泌态转化。结论:Nesfatin-1可能通过影响胃壁细胞超微结构变化抑制卡巴胆碱诱导的SD大鼠胃粘膜细胞的胃酸分泌。Objective： To investigate the effects and mechanisms of Nesfatin-1 on carbachol-stimulated gastric acid secretion in isolated rat gastric mucosal cells. Methods： Gastric mucosal cells of SD rats were isolated by enzymatically dispersion and treated by Nesfatin-1 （10^-1μmol/L） for different time or Nesfatin-1 （10^-1, 10^-2, 10^-3, 10^-4 μmol/L） for 0.5 h. Then 14C-Aminopyrine （14C-AP） accumulation was gauged as a measure of acid secretion to detect Nesfatin-1 on carbachol-induced gastric acid secretion in isolated rat gastric mucosal cells. Subsequently, the rat gastric mucosal cells were coincubated with Nesfatin-1 （10^-1μmol/L） and carbachol（100 μmol/L） for 0.5 h, transmission electron microscopywas used to observe the changes of parietal cells ultrastructure. Results： The carbachol-induced 14C-AP accumulation in isolated rat gastric mucosal cells were significantly inhibited by Nesfatin-1 （10^-1μmol/L） for 0.5 h and 1 h or Nesfatin-1（10^-1, 10^-2, 10^-3 μmol/L） for 0.5 h（P〈0.05）. Electronic microscope results showed that parietal cells transformed from resting to stimulated states in response to extracellular stimulation by carbachol （100 μmol/L）, which was inhibited by Nesfatin^-1 （10^-1μmol/L）. Conclusion： Nesfatin-1 could inhibit carbachol-induced gastric acid secretion in isolated rat gastric mucosal cells at least partly through influencing the ultrastructure of parietal cells.

关 键 词：NESFATIN-1 卡巴胆碱 胃酸 14C-AP 

分 类 号：Q95-3[生物学—动物学] R573[医药卫生—消化系统]