HERG钾通道在细胞容量调节中的作用  

The Contribution and Mechanism of the HERG Potassium Channels on the Regulation of Cell Volume

作　　者：梁晶[1] 刘惠彬[2] 李哲[3] 刘冬敏[1] 孙向菊[1] 吴禹蒙[1] 张鑫[1] 吴玉波[1] 

机构地区：[1]哈尔滨医科大学附属第四医院药学部,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第二医院药理学教研室,黑龙江哈尔滨150081 [3]哈尔滨医科大学机能学教研室,黑龙江哈尔滨150081

出　　处：《现代生物医学进展》2014年第32期6253-6256,6241,共5页Progress in Modern Biomedicine

基　　金：中国博士后科学基金项目(2012M520775)

摘　　要：目的:研究HERG钾通道在细胞容量调节中的作用,并探讨其作用机制。方法:全部试验应用稳定转染HERG-HEK293细胞和HEK293细胞。应用全细胞膜片钳技术记录HERG钾电流。结果:1当HERG-HEK293细胞处于低渗状态,指令电压为0 mV时,Istep增加60%(n=12,P<0.05);如指令电压为+30 mV,Itail增加72.1%(n=11,P<0.01),增大的HERG电流可被特异性HERG钾电流阻断剂Cisapride(100nM)抑制,Istep被抑制97.2%(n=6,P<0.01),Itail被抑制174.1%(n=6,P<0.01)。2在低渗状态,指令电压为0 mV时,容量调节性氯通道阻断剂尼氟灭酸(NFA,10 nmol/L)使Istep抑制46.2%(n=12,P<0.01),Itail抑制48.5%(n=11,P<0.01);给以容量调节性氯通道阻断剂DIDS 100μmol/L时,Istep抑制45.9%(n=12,P<0.01),Itail抑制51.1%(n=11,P<0.01)。结论:HERG通道部分参与调节性细胞容量下降过程。其参与的细胞容量调节与容量调节性氯通道的激活相伴随。Objective： To investigate the effect of HERG （Human ether-a-go-go related gene） potassium channels on the regulatory volume decrease and to reveal the possible mechanism. Methods： The stable HERG-transfected HEK293 cell line and HERG non-transfected HEK293 cell line were used for all the experiments, HERG potassium current was recorded in whole-cell patch-clamp techniques. Results： （1） When the HERG-HEK293 cells are exposed to the hypotonic solution and the test potential is 0mV, the Istep increased 60%（n=12, P〈0.05）; the Itail increased 72.1% （n=11, P〈0.01）; the increase currents can be inhibited by the specific blocker Cisapride （100nmol/L）,/step can be inhibited by 97.2% （n=6, P〈0.01） and the/tail can be inhibited by 174.1% （n=6, P〈0.01）. （2） Exposure to the hypotonic solution and in the presence of the volume regulated chloride channel blockers Nifiumic Acid （10 nmol/L）, the Istep can be inhibited by 46.2% （n=12, P〈0.01） and the Itail can be inhibited by 48.5% （n=11, P〈0.01）; In the presence of DIDS （100 μmol/L）, the Istep can be inhibited by 45.9% （n=12, P〈0.01） and Itail can be inhibited by 51.1%（n=11, P〈0.01）. Conclusinn： HERG potassium channels are partly involved in the process of the regulatory volume decrease, and the involvement followed the activation of the volume regulated chloride channel.

关 键 词：HERG钾通道 膜片钳 细胞容量 调节性细胞容量下降 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]