大鼠腹腔异位心脏移植模型建立及改进  

The Establishment and Improvement of Heterotopic Heart Transplantation Model in Rats

作　　者：王志远[1] 张丽丽[1] 顾金霞[1] 李学奇[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2014年第31期6019-6021,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81102236/H1006);黑龙江省博士后科研启动基金项目(LRB03-129)

摘　　要：目的:建立一种快速有效的大鼠腹腔异位心脏移植模型。方法:采用SD大鼠作为受体,Wistar大鼠作为供体,行同种异位腹腔心脏移植,术后给以CsA5 mg/kg/d灌服,心脏移植手术方法采用改良的Ono术式,观察改良的腹腔异位心脏移植各步骤所需时间、术后成功率及主要并发症发生率。结果:共建立40只大鼠异位心脏移植模型,手术成功率92.5%。动脉吻合时间12.5±2.3min,静脉吻合时间12.3±1.5 min,供心缺血时间37±3.5 min,受体血管阻断时间34.2±2.6 min,总手术时间90.2±4.8 min,出现的主要并发症为出血和供心复跳失败(各占5%、2.5%)。结论:改进的大鼠腹部异位心脏移植技术是一种简便、快速、有效、成功率高的模型制作方法。Objective： To establish a rapid and effective heterotopic heart transplant model in rats. Methods： Allogeneic abdominal heterotopic heat transplantation was performed to make the SD rats as recipients and Wistar rats as the donors. After surgery, the rats were given the CsA5 mg/kg/d by gavage. The surgery of Ono style was further optimized based on the abdominal heterotopic heart transplantation, the time for each step, success rate and main complications after surgery. Results： 40 rats＇ heterotopic heart transplantation models were established with the success rate of 92.5%, and the aortic anastomosis time was 12.5± 2.3 min, the pulmonary artery anastomosis time was 12.3 ± 1.5 min, total donor heart ischemia time was 37± 3.5 min, vessel occlusion time was 34.2±2.6 min, and total time surgery was 90.2± 4.8 min. The main complications were bleeding and/or resuscitation for heart failure which occurred to 5 % and 2.5 % of experimental rats, respectively. Conclusion： Improved abdominal heterotopic heart transplantation is a simple, rapid, effective and high success rate method of model establishment.

关 键 词：大鼠 异位心脏移植 动物模型 Ono术式 

分 类 号：Q95-3[生物学—动物学] R54[医药卫生—心血管疾病]