补肾活血汤对去卵巢大鼠心脏微血管内皮细胞形态及血清E_2、ET、NO、PGI_2、TXA_2含量的影响  

Effect of BSHXT on Endothelial Cell of Cardiac Microvessel and PGI_2, TXA_2, E_2, ET, NO in Ovariectomized Rats

作　　者：张玉芬[1,2] 王玉珍[1] 赵洁[3] 宋欢欢[1] 陈艳秋[1] 吴雷涛 李浩[1] 马静[1] 

机构地区：[1]第四军医大学西京医院中医科暨全军内科中心,陕西西安710032 [2]解放军第五三六医院内二科,青海西宁810003 [3]第四军医大学人体组织与胚胎学教研室,陕西西安710032

出　　处：《现代生物医学进展》2014年第31期6044-6049,6038,共7页Progress in Modern Biomedicine

基　　金：陕西省科学技术研究发展计划项目(2012K19-05-07)

摘　　要：目的:研究补肾活血汤对去卵巢大鼠冠脉微血管内皮细胞形态及功能的影响。方法:3月龄成年雌性大鼠随机分为8组,共计46只,分别为:正常组(NORM)、假手术组(SHAM),去卵巢组(OVX),补肾活血汤高、低剂量组(OVX/BHT-H、OVX/BHT-L)、17β-雌二醇干预组(OVX/ERT)。以透射电镜观察微血管内皮细胞细胞器变化;放射免疫法测定实验大鼠血清E2、ET、PGI2、TXA2的含量;采用硝酸还原酶法测定血浆NO含量。结果:与正常组相比,去卵巢组心肌及微血管内皮细胞细胞器破坏严重,中药组及17β-雌二醇干预组有所改善。与正常组相比,去卵巢组大鼠血浆中E2下降明显,有显著性差异;与去卵巢组相比,中药组大鼠E2明显上升,有显著性差异;与雌激素组相比无明显差异;与去卵巢组相比,中药组大鼠血浆ET-1值有所降低,其中以高剂量组最明显,中药组组大鼠的NO值稍有升高,但与正常组相比,没有显著性差异;中药组大鼠的TXA2值明显降低,其中以高剂量组最明显,中药组PGI2值升高,与正常组相比,有显著性差异,与17β-雌二醇干预组相比无显著性差异。结论:补肾活血汤能预防去卵巢后心肌微血管内皮细胞的形态和功能的破坏,其机制可能与其增加E2含量有关。Objective： To investigate the effect of BSHXT on the morphology and fimction of microvessel and endothelial cells in ovariectomized rats. Methods： Forty-six 3-month-old adult female rats were randomly divided into six groups： normal group（NORM）, sham operation group （SHAM）, ovariectomized group （OVX）, higher dose of BSHXT （OVX/BHT-H）, lower dose of BSHXT （OVX/BHT-L）, and ovariectomized＋17β estradiol intervening group （OVX/ERT）. The organelle changes ofmicrovascular endothelial cells were detected by transmission electron microscopy. In addition, the content of E2, ET, PGI2, TXA2 of rats were measured by radioimmunoassay, and the content of NO was measured by enzymatic determination. Results： The myocardial injury and the damage of organelle structure of microvascular endothelial cells in the model group and the oestrogen intervening group were more serious than those in the normal group, while it improved for the TCM group and the oestrogen intervening group. There was relationship between efficacy and doses. E2 in blood plasma of experimental rats in the model group decreased obviously compared with that in the normal group, while it increased significantly in the TCM group compared with that in the model group, but there was no obvious change compared in the Estrogen group. ET-1 in the TCM group decreased especially for the higher dose group compared with that in the model group. NO in the TCM group slightly increased but not obvious compared with that in the control group. TXA2 in the TCM group decreased significantly especially for the higher dose group, while PGI2 increased significantly compared with that in the control group. Conclusion： BSHXT can prevent the morphological and functional damage of microvessel and endothelial cells of ovariectomized rats. Consequently, cardiac insufficiency of ovariectomized rats can be prevented which may be related to the increase of E2 in blood plasma.

关 键 词：补肾活血汤 去卵巢大鼠 雌二醇 微血管 内皮细胞 

分 类 号：R541.9[医药卫生—心血管疾病]