富含半胱氨酸的酸性分泌蛋白与糖尿病肾病的相关性研究  

Correlation of secreted protein acidic and rich in cysteine with diabetic nephropathy

作　　者：李磊[1] 宋海燕[1] 刘锴[1] 朱莉莉[1] 安盟盟 王政[1] 

机构地区：[1]哈尔滨医科大学激素与内分泌疾病重点实验室哈尔滨医科大学附属第二医院内分泌代谢病科,150086

出　　处：《中华内分泌代谢杂志》2014年第9期741-746,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金（81370903）;黑龙江省科技计划项目（LC06C23）

摘　　要：目的 检测富含半胱氨酸的酸性分泌蛋白（secreted protein acidic and rich in cysteine,SPARC）在糖尿病肾病患者血清中的含量及在db/db糖尿病肾病小鼠肾脏组织中SPARC mRNA和蛋白的表达,探讨SPARC在糖尿病肾病发生发展中的作用.方法 采用夹心酶联免疫吸附法检测正常人、2型糖尿病患者（无肾病）、慢性肾功能不全患者（无糖尿病）以及糖尿病肾病患者血清SPARC水平;以RT-PCR、Western印迹法和免疫荧光染色法检测12周龄db/db小鼠肾脏组织的SPARC mRNA和蛋白表达.结果 糖尿病肾病患者血清中的SPARC含量明显高于正常组、2型糖尿病组及慢性肾功能不全组[（5.78±1.41对3.58±0.41、4.51±1.08和3.81±1.16）μg/L,P＜0.05或P＜0.01],2型糖尿病组高于正常组（P＜0.05）,2型糖尿病患者与慢性肾功能不全患者相比无明显差异,慢性肾功能不全患者与正常人相比无明显差异.db/db小鼠肾脏组织SPARC mRNA和蛋白表达明显高于对照组（P＜0.05）.结论 糖尿病肾病时长期高血糖导致肾脏病理改变的同时,肾脏组织产生和分泌的SPARC蛋白增加导致其血清浓度升高,可能参与糖尿病肾病病理改变的形成.Objective To investigate the serum concentrations of secreted protein acidic and rich in cysteine （SPARC） in patients with diabetic nephropathy and SPARC mRNA and protein expressions in renal tissue of db/db mice.Methods Serum SPARC levels in normal subjects and patients with type 2 diabetes mellitus （without diabetic nephropathy）,chronic renal failure （without diabetes mellitus）,and diabetic nephropathy were determined with enzyme-linked immunosorbent assay.RT-PCR,Western blot,and immunofluorescence were used to detect the mRNA and protein expressions of SPARC in renal tissue of db/db mice.Results The serum level of SPARC in diabetic nephropathy group was significantly higher than those in normal group,type 2 diabetes mellitus,and chronic renal failure group [（5.78 ± 1.41 vs 3.58 ±0.41,4.51 ± 1.08,and 3.81 ± 1.16） μg/L,P＜0.05 or P＜0.01].The SPARC level in the type 2 diabetes mellitus group was higher than that in normal group （P＜0.05）,but there was no difference between normal group and chronic renal failure.SPARC mRNA and protein levels in renal tissue of db/db mice were higher compared with the littermates （P ＜ 0.05）.Conclusions The long term hyperglycemic state in patients with diabetic nephropathy causes pathological change of renal tissue.Simultaneously,increased secretion of SPARC from renal tissue results in elevation of serum SPARC level and may play a role in pathological change of diabetic nephropathy.

关 键 词：富含半胱氨酸的酸性分泌蛋白 糖尿病肾病 胰岛素样生长因子 转化生长因子Β1 

分 类 号：R587.2[医药卫生—内分泌]