Biological and biochemical characterization of a red-eye mutant in Nilaparvata lugens （Hemiptera： Delphacidae）  

作　　者：Shu-Hua Liu Jing Yao Hong-Wei Yao Peng-Ling Jiang Bao-Jun Yang Jian Tang 

机构地区：[1]State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou [2]Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing [3]Institute of Insect Sciences, State Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China

出　　处：《Insect Science》2014年第4期469-476,共8页昆虫科学（英文版）

基　　金：We thank Professor Ze-Wen Liu of Nanjing Agricul- ture University and Researcher Jian-Li Wu of China National Rice Research Institute for their writing assis- tance of this article. This work was supported by Zhe- jiang Provincial Natural Science Foundation of China （LY 12C 14002）, National Natural Science Foundation of China （31201511） and National Basic Research Program of China （2010CB 126206）.

摘　　要：A red-eye colony was established in our laboratory in brown planthopper （BPH）, Nilaparvata lugens （Stal）, a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the wild-type （brown eye） and the mutant-type （red eye） in external characters. Genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg produc- tion and egg viability in the red-eye mutant colony were not significantly different from those in the wild-type BPH. Biochemical analysis and electronic microscopy examination revealed that the red-eye mutants contained decreased levels of both xanthommatin （brown） and pteridine （red） and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red-eye mutation. Our results indicate that the red-eye mutant gene （red） might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes.A red-eye colony was established in our laboratory in brown planthopper （BPH）, Nilaparvata lugens （Stal）, a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the wild-type （brown eye） and the mutant-type （red eye） in external characters. Genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg produc- tion and egg viability in the red-eye mutant colony were not significantly different from those in the wild-type BPH. Biochemical analysis and electronic microscopy examination revealed that the red-eye mutants contained decreased levels of both xanthommatin （brown） and pteridine （red） and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red-eye mutation. Our results indicate that the red-eye mutant gene （red） might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes.

关 键 词：biological characterization eye pigment genetic analysis Nilaparvatalugens pigment granule red-eye mutant 

分 类 号：S511[农业科学—作物学] S435.112.3