表达芯片筛选类风湿关节炎特异表达基因  

Screening for Human Rheumatoid Arthritis Markers using Expression Microarray

作　　者：岳龙涛[1] 张伟[2] 郑亚冰[1] 刘文波[1] 常晓天[1] 

机构地区：[1]山东大学山东省千佛山医院医学研究中心,济南250014 [2]济南市第二人民医院,济南250001

出　　处：《中国生物化学与分子生物学报》2014年第9期899-904,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点基础研究发展规划(973计划,No.2010CB529105)资助~~

摘　　要：为了寻找类风湿关节炎(rheumatoid arthritis,RA)新的特异性表达标记物,应用基因芯片对RA、骨关节炎(osteoarthritis,OA)和强直性脊柱炎(ankylosing spondylitis,AS)患者关节滑膜组织的基因表达谱进行比较,并采用实时定量PCR方法对芯片结果进行验证.全基因组表达芯片的结果显示,与OA滑膜组织及AS滑膜组织相比,在RA患者的滑膜组织中,CD38,ANKRD38,E2F2,CFDP1,CD7,ISG20和IL2RG基因表达异常;实时定量PCR结果证实,RA患者滑膜组织中,CD38、E2F2和IL2RG基因表达明显增高.The study was to find rheumatoid arthritis （RA）-specific gene by comparing the expression profiles of synovial membranes from patients with RA, osteoarthritis （OA） and ankylosing spondylitis （AS）. Illumina HumanHT-12 v4 Expression BeadChip was used to profile the global gene expression of patient synovial tissues and the results were verified by real-time PCR. By comparing the profiles, we identified the CD38, ANKRD38, E2F2, CFDP1, CD7, ISG20 and IL2RG genes as differentially expressed genes in RA synovial tissues. Increased expression of CD38, E2F2 and IL2RG were confirmed by qPCR. The CD38, E2F2 and IL2RG overexpression in synovial tissues were suggested to associate with RA as specific indexes.

关 键 词：类风湿关节炎 基因表达谱芯片 CD38 IL2RG E2F2 

分 类 号：Q33[生物学—遗传学]