LPS上调大鼠HIF-1和GLUT表达  

LPS up-regulates HIF-1 and GLUT expressions in rats

作　　者：严洁萍[1] 俞佳[1] 吴越[1] 吕良忠[1,2] 

机构地区：[1]浙江省人民医院药学部临床药学室,浙江杭州310014 [2]浙江中医药大学药学院药理教研室,浙江杭州310053

出　　处：《基础医学与临床》2014年第10期1367-1371,共5页Basic and Clinical Medicine

基　　金：浙江省自然科学基金(Y2100564)

摘　　要：目的 探讨脂多糖（LPS）致大鼠内毒素血症早期,葡萄糖转运体（GLUT）家族在脑、心和肝组织等表达水平及低氧诱导因子-1（HIF-1）调控的相关性.方法 雄性SD大鼠分为对照组（腹腔注射0.9％氯化钠注射液）和给药组（腹腔注射2 mg/kg LPS）,每组6只.测定给药后0～24h体温.ELISA法检测血清IL-1水平.RT-PCR法测定GLUT家族mRNA表达.Western blot法检测GLUT1和HIF-1蛋白表达.结果 在大鼠脑、心及肝等组织中,GLUT1和GLUT4均有表达;GLUT2在脑和肝组织中有表达;GLUT3仅在脑组织特异性表达.LPS作用24h可引起大鼠体温升高,血清IL-1水平上调（P＜0.05）.LPS可上调脑组织GLUT1 mRNA水平和蛋白翻译水平（P＜0.01）;同时LPS可促进脑组织HIF-1蛋白稳定表达（P＜0.001）.结论 LPS促进大鼠HIF-1稳定表达,上调GLUT1表达及调控糖代谢.Objective Characterization of glucose transporter （GLUT） family and potential role of HIF-1 in lipopolysacchride （LPS）-induced endotoxemic rats is investigated in endotoxemic rats.Methods SD rats were treated with a single dose injection （i.p.） of LPS（2 mg/kg）,and rats in control group were given equal amount of NaCl （0.9％） （n =6）.The body temperature was detected after LPS treatment for 0 ～ 24 h.IL-1 release was evaluated by ELISA assay.GLUT mRNA expression was determined by RT-PCT assay.HIF-1 and GLUT1 protein expression were examined.Results LPS enhanced rats＇ body temperature and IL-1 release in serum （P ＜ 0.05）.GLUT1 and GLUT4 expressed in brain,heart and liver.GLUT2 expressed in brain and liver.GLUT3 mainly expressed in brain.LPS increased GLUT1 mRNA and protein expression in the brain （P ＜0.01）.HIF-1 kept stability in the brain of LPS-challenged rats （P ＜ 0.001）.Conclusions LPS induces GLUT1 overexpression and improve HIF-1 stability in the brain of the endotoxemic rats.It indicates that HIF-1-induced GLUT1 upregulation is a potential pathway to regulate glucose metabolism in LPS-challenged rats.

关 键 词：葡萄糖转运体 低氧诱导因子-1 脂多糖 SD大鼠 

分 类 号：R965[医药卫生—药理学]