复方四季青软膏的制备及质量控制  

Preparation and quality control of compoundointment for Sijiqing

作　　者：涂金兰[1] 蔡绚[1] 尹航[1] 杨信定[1] 何雨[2] 石白冰 

机构地区：[1]贵阳医学院附属医院药剂科,贵州贵阳550004 [2]贵阳医学院药学系,贵州贵阳550004

出　　处：《中国医药科学》2014年第18期29-33,共5页China Medicine And Pharmacy

基　　金：贵州省科技厅中药现代化科技产业研究开发专项项目(黔科合中药字[2011]5011号)

摘　　要：目的鉴别复方四季青软膏中各主药的真伪;研究复方四季青软膏的制备方法;建立复方四季青软膏含量的控制方法。方法以薄层色谱法鉴别四季青叶、大黄、紫草;以紫外分光光度法测定紫草中左旋紫草素的含量。结果左旋紫草素的乙醇溶液在5.64～33.87μg/mL范围内,A与C呈良好线性关系,r=0.9999（n=6）,平均回收率为98.80%,RSD（%）=0.88（n=6）。结论该方法准确、简便,重现线好,可用于复方四季青软膏中紫草含量的控制。Objective To identify the authenticity of the main drug in compound Sijiqing ointment; to study the preparation method of compound Sijiqing ointment; to establish a method to control the content of compound Sijiqing ointment. Methods Using the TLC method to identify the four leaf, rhubarb, Radix Arnebia; Determination of the content of levo shikonin in shikonin by UV spectrophotometry. Results The ethanol solution of levo shikonin was in the range of 5.64-33.87μg/mL, showed a good linear relationship between A and C, r=0.9999（n=6）, the average recovery was 98.80%, RSD（%） =0.88（n=6）. Conclusion The method is accurate, simple, good reproducible line, It can be used to control the content of shikonin in compound ointmentin Sijiqing.

关 键 词：复方四季青软膏 含量测定 左旋紫草素 

分 类 号：R927[医药卫生—药学]