泥鳅对饲料中添加转融合基因Cry1Ab/Ac水稻花粉的响应  

Response of Misgurnus anguillicaudatus to feed with transgenic Cry1Ab/Ac rice pollen added

作　　者：李菲[1] 刘雨芳[1] 孙远东[1] 莫书银 肖璐[1] 谭树华[1] 刘文海[1] 桂芳艳 戈峰[2] 

机构地区：[1]湖南科技大学生命科学学院,园艺作物病虫害治理湖南省重点实验室,湖南湘潭411201 [2]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室,北京100101

出　　处：《生态学杂志》2014年第10期2727-2732,共6页Chinese Journal of Ecology

基　　金：国家转基因生物新品种培育重大专项(2012ZX08011002);湖南科技大学优秀学位论文培育项目(S120029)资助

摘　　要：以转Cry1Ab/Ac抗虫水稻华恢1号(HH1)花粉为添加剂,配制4种不同花粉含量(1%、5%、10%、20%)的饲料,以非转基因亲本水稻明恢63(MH63)花粉为对照,常规泥鳅饲料(不添加花粉)为阴性对照,投喂100 d,通过泥鳅的生长指标和肝脏生理酶活性评价转Cry1Ab/Ac抗虫水稻HH1号花粉对泥鳅的安全性。结果表明,在饲料中分别添加相同比例的HH1与MH63花粉,两组泥鳅的增重率、脏体比和肝体比指数均无显著差异,同时对泥鳅的肝脏抗氧化保护酶SOD、CAT活性与解毒酶GST活性也均无显著影响。饲料中花粉含量为20%时,在饲养100 d后,转Cry1Ab/Ac水稻花粉对泥鳅的生长性能与肝脏生理酶代谢功能没有明显影响。因此,转Cry1Ab/Ac抗虫水稻HH1号花粉对泥鳅无明显的生态风险。As an additive, the pollen of transgenic CrylAb/Ac insect-resistant rice （ HH1 ） was added to the normal feed of Misgurnus anguillicaudatus, which made the artificial feed containing 5%, 10% and 20% pollen, respectively. M. anguillicaudatus were cultured in laboratory for 100 days fed on one of the artificial feeds mentioned above. Feed with the pollen of non- CrylAb/Ac parent rice （MH63） added was used as a control, while the regular feed without any pollen was used as a negative control. The ecological safety of transgenic CrylAb/Ac rice pollen on M. anguilllcaudatus was evaluated by testing the growth performance and activities of physio- logical enzymes in liver of M. anguillicaudatus. The results showed that no significant differences were found in the weight gain rate, viscera somatic index and liver somatic index of M. anguillicaudatus feeding transgenic CrylAb/Ac rice pollen compared to that in the control groups. The activities of two antioxidant protective enzyme, SOD and CAT, and detoxification enzyme GST of M. anguillicaudatus had no significant difference either. With up to 20% pollen content of trans- genic CrylAb/Ac rice in feed, no obvious influences on the growth performance and physiological metabolism of liver function of M. anguillicaudatus were found after feeding 100 days. The results suggested that the pollen of transgenic on M. anguillicaudatus. CrylAb/Ac rice would have no obvious ecological risk

关 键 词：生长性能 SOD CAT GST 

分 类 号：S966.4[农业科学—水产养殖]