土壤四环素污染对蚯蚓生长发育、抗氧化防御系统及基因表达的毒性效应  

Ecotoxicological effects of tetracycline on the growth,antioxidant defense system and gene expression of earthworm Eisenia foetida in soil

作　　者：纪占华[1,2] 安婧[1] 肖明月[3] 魏树和[1] 台培东[1] 律泽[4] 

机构地区：[1]中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049 [3]辽宁石油化工大学,辽宁抚顺113001 [4]东北大学,沈阳110004

出　　处：《生态学杂志》2014年第10期2767-2774,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(40930739;41001340;21277150;31370523);国家环境保护公益性行业科研专项项目(201209030);辽宁环境科研教育123工程项目(CEPF2012-123-2-1)资助

摘　　要：采用室内模拟实验,研究了土壤四环素污染对赤子爱胜蚓（Eisenia foetida）生长发育、抗氧化系统及基因表达的毒性效应。结果表明：与对照组相比,四环素暴露初期（≤14d）,蚯蚓体重及可溶性蛋白（SP）含量变化不显著;随着暴露时间的延长及四环素浓度的增加,蚯蚓体重呈现下降趋势,SP含量显著上升。1～100 mg·kg-1四环素胁迫下,蚯蚓体内抗氧化防御系统遭到破坏,暴露14 d后蚯蚓体内SOD、POD和CAT活性均有不同程度的上升。DDRT-PCR试验结果显示,1～100 mg·kg-1四环素胁迫下,暴露28 d后的蚯蚓基因表达发生变化。研究表明,四环素对蚯蚓具有慢性生态毒性效应,可以综合运用抗氧化酶和基因表达等生物标志物评价四环素的生态毒性。A simulated experiment in laboratory was conducted to study the ecotoxicological effects of tetracycline （TC） on the growth, antioxidant defense system and the gene expression of earthworm Eisertiafoetida in soil. The results showed that, in the early exposure （ ≤14 d）, the weight and soluble protein （SP） of earthworm stressed by 1-100 mg· kg-1 TC had no significant difference from the control. However, with the increase of TC concentration and the prolonging of exposure time, the weight of earthworm decreased and the content of SP increased. The balance of the antioxidant system was destroyed by 1-100 mg ·kg-1 TC with the activities of SOD, POD and CAT increased to varying degrees after a 14 d exposure. Moreover, the results of DDRT-PCR showed that 1-100 mg · kg-1 TC affected the gene expression of earthworm after a 28 d exposure. It was suggested that TC had chronic ecotoxicologieal effects on earthworm and the biomarkers such as antioxidant defense enzyme and the gene expression can be used to estimate the ecotoxi- cological effects of TC.

关 键 词：四环素 蚯蚓 土壤污染 生态毒性效应 

分 类 号：X53[环境科学与工程—环境工程] X171.5