甘露醇保存甘薯种质试管苗的效果  

Effect of Mannital on the Conservation of Sweet Potato Germplasm in Vitro Plantlets

作　　者：黄萍[1] 马朝宏[2] 颜谦[1] 

机构地区：[1]贵州省马铃薯研究所,贵州贵阳550006 [2]贵州省农业科学院知识产权办,贵州贵阳550006

出　　处：《天津农业科学》2014年第10期92-94,共3页Tianjin Agricultural Sciences

基　　金：贵州省现代农业马铃薯产业体系建设项目(GZCYTX2013-0401)

摘　　要：以贵州仁怀地方甘薯品种脱毒试管苗为试验材料,研究不同浓度的甘露醇（2%-10%）对试管苗保存效果的影响。结果表明：甘露醇可抑制试管苗的生长,延长保存时间,其中以6%-8%的浓度最为理想,可保存试管苗11个月,成活率达35%,且不影响试管苗继代。Virus-free plantlets of local variety of sweet potato was taken as experimental material and the multiplication culture medium supplemented with different concentration mannital（2%-10%） were used as the regard proliferation medium to study the effect of different concentration mannital on the growth and conservation of tube plantlets. The results showed that mannital could not only inhibit the growth of the tube plantlets but also prolong the conservation period. When the concentration of mannital was 6%-8%,the plantlets could be preserved at least eleven months and the survival rate was 35%,but had no significant effect on subculture.

关 键 词：甘薯种质 试管苗 甘露醇 

分 类 号：S531[农业科学—作物学]