以Ypt1基因序列为靶标的辣椒疫病菌快速分子检测  

Rapid molecular detection of Phytophthora capsici based on its Ypt1 gene

作　　者：李毛毛[1] 赵伟[2] 汪涛[2] 谷雨[2] 高智谋[1] 戚仁德[2] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031

出　　处：《植物病理学报》2014年第5期546-551,共6页Acta Phytopathologica Sinica

基　　金：国家公益性行业(农业)科研专项(201303018);安徽省农科院院长青年创新基金项目(11B1111)

摘　　要：辣椒疫霉（Phytophthora capsici）是一种重要的植物病原卵菌，寄主范围广泛，除了辣椒以外，还可以侵染番茄、茄子及多种瓜类，造成疫病，危害作物生产。随着设施农业的大规模发展，蔬菜种植面积日益扩大．该菌所致病害及造成的经济损失逐年加重。对辣椒疫霉菌进行早期检测可以及时采取防治措施，控制病害进一步扩展。PCR primers were designed based on the sequence of Ras-related protein gene （ Yptl ） of P. capsici. According to the multiple sequence alignment, Yptl has the sufficiently polymorphic intron region for the development of P. capsici-specific primers （PcYptlF/PcYptlR）. One primer pair was developed which can amplify one P. capsici-specific fragment of 156 bp. Using the primer pair, the P. capsici infected plants and soils were detected. Additionally, Yptl has an appropriate region for the development of Phytophthora genus-specific primers （YptlF/YptlR）, which can amplify a fragment of about 540 bp from 14 different Phytophthora specices and a fragment of about 350 bp in Pythium species, with no amplification from fungal species. By PCR optimization using P. capsici genomic DNA, the detection sensitivities of 10 pg and 10 fg DNA were achieved in standard PCR （PcYptlF/PcYptlR） and nested PCR （YptlF/YptlR and PcYptlF/PcYptlR）, respectively. The developed primers were proved to be efficient in detection of Phytophthora pathogens from diseased plant tissues and residues in soils.

关 键 词：基因 辣椒疫病菌 快速分子检测 植物研究 

分 类 号：Q78[生物学—分子生物学] S432.44[农业科学—植物病理学]