利用PCR和RT-PCR检测草莓镶脉病毒的比较研究  

Comparative study on detection of Strawberry vein banding virus by PCR and RT-PCR

作　　者：宋成林[1] 官春月 代红艳[1] 刘月学[1] 马跃[1] 李贺[1] 张志宏[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110866

出　　处：《植物病理学报》2014年第5期552-555,共4页Acta Phytopathologica Sinica

基　　金：国家公益性行业(农业)科研专项(201003064)

摘　　要：草莓镶脉病毒（Strawberry vein banding virus，SVBV）是一种双链DNA病毒，属于花椰菜花叶病毒属，病毒粒子呈球形，直径为45nm[1]。SVBV基因组全长7876bp，包括7个开放阅读框，其编码的蛋白分子量分别为37．8、18．3、16．6、56．0、81．1、59．0和12．6kDa心]。Strawberry vein banding virus （SVBV） is a double-stranded DNA virus. In the study, real-time reverse transcription-polymerase chain reaction （RT-PCR） was used to analyze the transcript level of SVBV coat protein gene （cp） in strawberry young leaves, old leaves and fruits at five different developmental stages. The results showed that the cp was expressed in all tested organs. However, its transcript level was higher in old leaves and lower in young leaves. Furthermore, PCR was compared with RT-PCR for detecting SVBV in old leaves of strawberry. Non-specific amplicons could be found in PCR products while only the specific amplicon was generated in RT-PCR products. In order to avoid the accumulation of non-specific amplicons in PCR products, the comparison between Taq DNA polymerase and hot-start Taq DNA polymerase was carried out. There were no non-specific amplicons in PCR products amplified with hot-start Taq DNA polymemse, and the repeatability of the assays was good. This study laid a foundation for making the protocol of molecular detection of SVBV in strawberry.

关 键 词：草莓镶脉病毒 RT-PCR检测 PCR 植物研究 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]