云南蔗区发现由植原体引起的检疫性病害甘蔗白叶病  

A quarantining sugarcane white leaf disease caused by phytoplasma found in sugarcane field in Yunnan

作　　者：李文凤[1] 王晓燕[1] 黄应昆 单红丽[1] 申科[1] 罗志明[1] 张荣跃[1] 尹炯[1] 

机构地区：[1]云南省农业科学院甘蔗研究所云南省甘蔗遗传改良重点实验室,开远661699

出　　处：《植物病理学报》2014年第5期556-560,共5页Acta Phytopathologica Sinica

基　　金：现代农业产业技术体系建设专项资金资助(CARS-20-2-2);云南省现代农业产业技术体系建设专项资金资助

摘　　要：甘蔗白叶病（Sugarcane white leaf，SCWL）是由植原体引起的甘蔗重要病害之一。1954年首次在泰国北部南邦府发现。我国台湾1958年报道了该病的发生情况[1]。该病在印度、巴基斯坦、斯里兰卡、日本、老挝、缅甸、越南、菲律宾、泰国等地均有大面积发生，造成甘蔗和制糖产业巨大经济损失[2]。By used of nested-PCR, the 16S rDNA sequence of phytoplasma associated with sugarcane white leaf （SCWL） in 48 suspected SCWL samples from Baoshan and Lincang of Yunnan were amplified with two primer pairs MLOX/MLOY and P1/P2. The sequencing showed that the fragment size of 17 Baoshan suspected SCWL samples were all 210 bp and their sequences were all identical （GenBank： KC662509） ; the fragment size of 10 Lincang suspected SCWL samples were all 202 bp and their sequences were all identical （GenBank： KF431837）. The Blast result indicated that the sequences obtained in this study were derived from the 16S-23S ISR intergenic spacer region of phytoplasma that causes SCWL and were highly homologous （99. 05%-100% similarity） to the corresponding genome region registered in GenBank. Plant height, stalk diameter, millable stalk rate and single stalk weight were significantly reduced by infection of SCWL, which caused destructive damage to sugarcane.

关 键 词：检疫性病害 甘蔗 白叶病 植物研究 

分 类 号：S666[农业科学—果树学]