低聚壳聚糖对福瑞鲤生长、非特异性免疫及抗氧化指标的影响  

Effects of dietary chitosan-oligosaccharide on the growth performance,nonspecific immunity and antioxidant parameters of FFRC Strain Common Carp

作　　者：张严伟[1,2] 陈跃平[1] 李嫔[1] 李向飞[1,3] 刘文斌[1,3] 周岩民[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]江苏省农垦集团有限公司,江苏南京210008 [3]江苏省水产动物营养重点实验室,江苏南京210095

出　　处：《上海海洋大学学报》2014年第5期726-732,共7页Journal of Shanghai Ocean University

基　　金：现代农业产业技术体系(nycytx-49-21)

摘　　要：以初始体重(33.90±0.11)g的福瑞鲤(FFRC Strain Common Carp,Cyprinus carpio)为研究对象,在基础日粮中分别添加0(对照组)、25、50和100 mg/kg低聚壳聚糖,于室内可控温循环水养殖系统中进行35 d的实验,研究了低聚壳聚糖对福瑞鲤生长、非特异性免疫及抗氧化指标的影响。结果表明,与对照组相比,低聚壳聚糖对福瑞鲤生长性能、肌肉成分无显著影响(P>0.05);各实验组血浆碱性磷酸酶活力显著提高(P<0.01);各实验组血浆总铁结合力水平、补体C3和C4含量有一定升高(P>0.05);25 mg/kg实验组脾脏NO水平极显著提高(P<0.01);50(P<0.05)和100mg/kg(P<0.01)实验组脾脏髓过氧化物酶活力均有显著提高;50mg/kg实验组血浆超氧化物歧化酶活力显著升高且丙二醛水平显著降低(P<0.05)。研究表明,饲料中添加适量的低聚壳聚糖对福瑞鲤的非特异性免疫和抗氧化功能有明显的增强作用。实验条件下,低聚壳聚糖添加量为50 mg/kg较适宜。This study was conducted to investigate the effects of chitosan-oligosaccharides on the growth performance,nonspecific immunity and antioxidant parameters of FFRC Strain Common Carp（ Cyprinus carpio）. The fish（ mean initial weight ： 33. 90 ± 0. 11 g） were randomly divided into 4 groups with 5replicates per group and 15 fish per replicate. The fish were fed with either basal diet（ control group） or basal diet supplemented 25,50 and 100 mg /kg chitosan-oligosaccharides for 35 d,respectively. The results showed that dietary supplementation of chitosan-oligosaccharides did not significantly affect the growth performance and muscle composition of fish compared with the control group（ P 〉 0. 05）. As expected,dietary chitosanoligosaccharide supplementation significantly enhanced the activities of plasma alkaline phosphatase（ AKP）.The similar effects were also observed on the concentrations of plasma complement 3（ C3） and complement 4（ C4） as well as the content of total iron binding capacity（ TIBC） in plasma（ P 〉 0. 05）. In addition,dietary chitosan-oligosaccharide supplementation at 25 mg /kg also significantly enhanced the content of nitric oxide（ NO） in spleen. The activity of myeloperoxidase（ MPO） in spleen was promoted in fish fed chitosanoligosaccharide at 50（ P 〈 0. 05） and 100 mg /kg（ P 〈 0. 01）. Dietary 50 mg /kg chitosan-oligosaccharide supplementation significantly increased superoxide dismutase（ SOD） level and decreased the maleic dialdehyde（ MDA） content in plasma（ P 〈 0. 05）. Based on the results above,it was indicated that chitosan-oligosaccharides could effectively improve nonspecific immunity and antioxidant parameters of FFRC Strain Common Carp（ Cyprinus carpio） and the optimum level of 50 mg /kg exerted the best effects on FFRC Strain Common Carp in the present study.

关 键 词：低聚壳聚糖 福瑞鲤 生长性能 非特异性免疫 抗氧化指标 

分 类 号：S963.7[农业科学—水产养殖]