嗅痛灵嗅剂的质量标准研究  

Determination of Herba asari lignin in Xiutongling Snuff by HPLC

作　　者：陈智伟[1] 黄庆宝[1] 付文洋[1] 徐汉明[1] 

机构地区：[1]广东深圳市中医院,518033

出　　处：《内蒙古中医药》2014年第25期84-85,共2页Inner Mongolia Journal of Traditional Chinese Medicine

摘　　要：目的：建立HPLC测定嗅痛灵嗅剂中细辛木脂素含量的方法。方法：采用薄层色谱法鉴别嗅痛灵嗅剂中木香、细辛;采用HPLC对制剂中细辛木脂素进行含量测定,色谱柱：GL Inertsil ODS-SP色谱柱（5μm,150mm×4.6mm）;流动相：乙腈：水溶液梯度洗脱;流速：1ml/min;检测波长：287nm;柱温：40℃。结果：木香、细辛的薄层鉴别斑点清晰、专属性强;细辛木脂素在15.16744～75.8372μg呈良好线性关系。（r=9999,n=5）,平均回收率为97.88%,RSD%为1.18%（n=9）。结论：本研究建立的质量标准方法操作简单、专属性强、重现性好、结果准确可靠。Objective To estai）lish a method for the determination of HPLC concentrations of lignan olfactory pain spirit snuff. Methods： TLC method of olfactory pain spirit snuff in woody, asarum; using HPLC to preparation of Herba asari lignin content determination, chro- matographic column： GL Inertsil ODS-SP column （5μm, 150mm× 4.6mm）; mobile phase： aeetonitrile： water solution gradient elution; flow rate： Iml/ rain; the detection wavelength： 287nm; column temperature： 40 ℃. Result： The TLC spots Radix Aueldandiae, asarum clear, strong specificity; Herba asari lignin in 15.16744 - 75.8372μg showed a good linear relationship. （r=9999, n=5）, the average re- covery was 97.88%, RSD% was 1.18% （n=9）. Conclusion： The quality standard of the method has the advantages of simple operation, strong specificity, good reproducibility, and the result is accurate and reliable.

关 键 词：嗅痛灵嗅剂 细辛木脂素 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]