miR-10b对人正常滋养细胞浸润能力的影响  

Effect of miR-10b on the invasion of human trophoblast cell

作　　者：杨洋[1] 吴胜军[1] 雷蕾[1] 

机构地区：[1]西安医学院附属医院妇科,西安710077

出　　处：《山西医科大学学报》2014年第9期791-794,902,共5页Journal of Shanxi Medical University

基　　金：陕西省教育厅科研基金资助项目(12JK0770);西安医学院附属医院科研基金资助项目(XYFY11-05)

摘　　要：目的研究微小RNA-10b(microRNA-10b,miR-10b)对人正常滋养细胞系(HTR-8/SVneo)细胞浸润能力的影响。方法使用miR-10b前体分子转染人正常滋养细胞系,共分为三组:miR-10b过表达组、miR-10b抑制组及pre-miRNA阴性对照组(NC);RT-PCR检测转染后各组细胞中miR-10b在mRNA水平的表达;Tanswell小室技术检测转染后各组细胞浸润能力的变化。结果 RT-PCR结果显示过表达组、抑制组分别与阴性对照组对比,miR-10b过表达与抑制水平显著,差异具有统计学意义(P<0.05);Tanswell小室检测结果显示,过表达组细胞浸润能力有所升高,差异具有统计学意义(P<0.05)。结论过表达miR-10b可以显著影响滋养细胞的浸润能力,证明miR-10b的表达水平与滋养细胞的细胞功能正常与否有一定关联性。Objective To investigate the effect of miR-10b on the invasion capacity of normal trophoblast cell line（HTR-8/SVneo）.Methods The synthesized miR-10b was transfected into HTR-8 cells.The experiment included miR-10b overexpression group,miR-10b inhibition group and pre-miRNA untransfection group.Reverse transcription PCR（RT-PCR） was used to detect the expression of miR-10b in three groups.The invasive capacity of HTR-8 cells in three groups was determined by Transwell assay.Results RTPCR showed that the cells transfected with mature type miR-10b had significantly higher expression of miR-10b than the untransfection cells（P ＜ 0.05）.Transwell results showed that compared with untransfection group,the cell invasion capacity declined in overexpression group （P ＜ 0.05）.Conclusion Overexpression of miR-10 b could affect invasion capacity of normal trophoblast cell line（HTR -8/SVneo）,and the expression of miR-10b maybe has some relationship with the cell capacity of normal trophoblast cell line.

关 键 词：微小RNA 子痫前期 滋养细胞 浸润 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]