盐度对脊尾白虾蚤状幼体Na^+/K^+-ATPase和HSP基因表达的影响  

Effects of salinity on the mRNA expression of Na^+/K^+-ATPase and HSP in the ridgetail white prawn,Exopalaemon carinicauda

作　　者：沈盎绿[1] 蒋科技[1] 沈新强[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室,上海200090

出　　处：《海洋环境科学》2014年第5期693-698,共6页Marine Environmental Science

基　　金：国家重点基础研究发展规划项目(2010CB429005)

摘　　要：通过室内模拟实验,研究了不同梯度的盐度胁迫对脊尾白虾蚤状幼体的影响,记录了不同时间段幼体的死亡率,采用荧光定量PCR技术,分析了3个相关基因的表达情况。实验结果如下:盐度为14和28处理组中脊尾白虾蚤状幼体活动正常,过低(2,5和8)或过高(42)的盐度处理短时间内都会导致脊尾白虾蚤状幼体死亡;盐度为14和28这两组试验中脊尾白虾蚤状幼体的钠钾ATP酶(Na+/K+-ATPase)基因表达水平随着处理时间的延长略有上升,但是整体来说变化幅度不大,盐度为8处理组中前6 h Na+/K+-ATPase基因表达水平变化不大,第12 h时显著升高;盐度为8和28处理组中脊尾白虾蚤状幼体HSP70在2 h后就显著上升,盐度为28处理组中脊尾白虾上升幅度相比之下较小,盐度为14处理组中脊尾白虾HSP70在整个实验期间变化不大;脊尾白虾蚤状幼体体内热休克蛋白90(HSP90)对盐度的胁迫响应趋势跟HSP70基因类似,只是变化程度有所差异。总的来说,大型水利工程的兴建会导致河口地区盐度的异常变化,进而影响河口地区脊尾白虾蚤状幼体正常生活,这3个基因的表达水平变化可以作为河口盐度变化的生物标志物。The mRNA expression of Na ^＋/K^＋ -ATPase, heat shock protein families, HSP70 and HSP90, in Exopalaemon. carinicauda was investigated by real-time quantitative PCR stressed by the different gradient of salinity （low salinity water, LSW： 2, 5 and 8; brackish water, BW： 14; seawater, SW. 28; and high salinity water, HSW： 42）. The results showed that there were no death individual of the zoea of E. carinicauda in 14 and 28 treatment groups, and there were no living individual in lower salinity （2, 5 and 8） and higher salinity （42）. The mRNA expression of NKA increased slightly in 14 and 28 treatment groups and increased significantly in 8 treatment groups at 12 h. The mRNA expression of HSP70 increased significantly in 8 and 28 treatment groups at 2 h, but the amplitude of HSP 70 in 28 was smaller than in 8, and the mRNA expression of HSP70 in 14 was stable during 48 h. The rule of mRNA ex- pression of HSP90 was similar to that of HSP70. In general, such large-scale water conservancy projects could lead to abnormal salinity change in estuary area, which will affect the normal life of ridgetail white prawn early life stage in return; the three gene expression level changes can be used as biomarkers of estuary salinity changes.

关 键 词：脊尾白虾 盐度 钠钾ATP酶 热休克蛋白70 热休克蛋白90 

分 类 号：Q178.53[生物学—水生生物学]