中国北方健康人群血清25羟维生素D_3和25羟维生素D_2水平  

Serum 25-hydroxy vitamin D_3 and 25-hydroxy vitamin D_2 levels in healthy population from north China

作　　者：方慧玲[1] 禹松林[1] 韩建华[1] 秦绪珍[1] 李士军[2] 王亮[3] 程倩[1] 侯立安[1] 程歆琦[1] 夏良裕[1] 邱玲[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]大连医科大学附属第一医院检验科,大连116011 [3]新疆医科大学第一附属医院检验科,乌鲁木齐830054

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2014年第3期199-205,共7页Chinese Journal Of Osteoporosis And Bone Mineral Research

基　　金：国家临床重点专科项目;国家高技术研究发展计划(863计划)(2014AA022304)

摘　　要：目的：分析中国北方人群血清25羟维生素D3（25OHD3）和25羟维生素D2（25OHD2）水平。方法采用同位素稀释超高压液相色谱-串联质谱法（ID-UPLC/MS/MS）对2013年在北京、大连、乌鲁木齐3个城市征集的1540名表观健康志愿者[男性744人，女性796人，平均年龄（42.0±14.0岁）]进行血清25OHD3和25OHD2测定。同时分别应用偶氮Ⅲ砷法、磷钼酸紫外比色法、酶化学发光法检测志愿者血清钙（Ca）、磷（P）及全段甲状旁腺素（iPTH）水平。使用SPSS17.0进行统计学分析，P＜0.05表示差异有统计学意义。结果受试人群血清总25OHD平均值为（18.80±6.65）ng/mL。3个城市受试人群血清总25OHD含量差异有统计学意义（F＝97.57，P＜0.01），大连市受试者血清总25OHD水平[（21.72±6.47）ng/mL]显著高于乌鲁木齐市和北京市受试者[（18.44±6.64）ng/mL、（16.34±6.12）ng/mL]（均P＜0.01）；男性血清25OHD水平[（20.57±6.63）ng/mL]显著高于女性[（17.14±6.24）ng/mL]，（P＜0.01）；30～39岁年龄组25OHD含量显著低于其他年龄组（均P＜0.05）。3省受试人群血清总25OHD缺乏率为60.06％，大连市受试者血清总25OHD缺乏率（41.51％）显著低于乌鲁木齐市（64.87％）和北京市（73.76％），（均P＜0.01）。3个城市57例受试者（3.70％）血清25OHD2检测均值为（6.25±3.76）ng/mL。在57例检测25OHD2受试者中，25OHD2显著提高了血清总25OHD水平，使该人群血清25OHD缺乏率降低43.85％。血清25OHD水平与iPTH相关系数为-0.283（P＜0.01）。结论3个城市受试人群血清25OHD含量存在明显的地域、性别和年龄差异。健康人群中血清25OHD主要为25OHD3。北方地区人群血清25OHD水平普遍较低。Objective The aim of this study is to investigate the vitamin D status of healthy population in three northern Chinese cities .Methods We recruited 1540 apparently healthy volunteers in Beijing , Dalian and Urumqi with an average age of 42.0 ±14.0 years old （men 744, women 796）.Both 25OHD3 and 25OHD2 were measured with ID-UPLC/MS/MS method.Serum Ca, P and iPTH were obtained with Azo Ⅲ arsenic method, Phosphomolybdic acid ultraviolet colorimetry method , and Enzyme chemiluminescence method , respectively .Results Total serum 25 OHD con-centration was 18.80 ±6.65 ng/mL in the studied population .There were significant differences in serum 25OHD levels among the three cities （P〈0.01）.Subjects from Dalian had highest serum 25OHD level compared to Urumqi and Bei-jing （21.72 ±6.47 ng/mL vs 18.44 ±6.64 ng/mg vs 16.34 ±6.12ng/mL, P〈0.01）;Serum 25OHD level in men was significantly higher than that in women （20.57 ±6.63 ng/mL vs 17.14 ±6.24 ng/mL, P〈0.01）;Serum 25OHD level in subjects aged 30-39 was lower than that in other age groups （P〈0.05）.The total serum 25OHD deficient rate in the population was 60.06% and subjects in Dalian had the lowest serum 25OHD deficient rate among the three cities （41.51%vs 64.87%vs 73.76%, P〈0.01）.About 3.70% subjects had quantitative 25OHD2 with an average serum level of 6.25 ±3.76 ng/mL.The serum 25OHD deficient rate in subjects with quantitative 25OHD2 was 43.85%.We observed a significant increase in the deficient rate of serum 25OHD when serum 25OHD2 was removed from serum total 25OHD in those subjects （43.85%vs 85.96%, P〈0.01）.Conclusion Serum 25OHD level has significant difference in age, sex and regions among healthy individuals in north China .Serum 25OHD3 is the main part of 25OHD in healthy population .Vitamin D deficiency is prevalent in north China .

关 键 词：血清25羟维生素D 血清25羟维生素D3 超高压液相色谱-串联质谱 25OHD缺乏 

分 类 号：R681[医药卫生—骨科学]