诱导食用真菌代谢生产木聚糖酶的研究  

Screening of Xylanase-producing Edible Fungi by Xylan Induction

作　　者：邢振楠 臧海莲[2] 王丹丽 范冬茹 

机构地区：[1]伊春林业科学院,黑龙江伊春153000 [2]东北农业大学

出　　处：《中国林副特产》2014年第5期8-12,共5页Forest By-product and Speciality in China

摘　　要：以木聚糖为诱导底物,对66株大型食用真菌进行液体发酵诱导培养,采用超薄层琼脂板扩散法和DNS测定法进行初筛、复筛,最终筛选出3株产木聚糖酶较高的菌株。检测单因素结合正交试验,最终确定黑木耳LK8、玉田平菇、香菇236经木聚糖诱导,产木聚糖酶的最佳培养时间、pH值和底物木聚糖浓度分别为20d、7.5和1.0g/100mL;25d、7.0和1.0g/100mL;25d、5.0和0.75g/100mL,最佳条件组合时的酶活分别为:600.6IU/mL,672.5IU/mL,345IU/mL。并且,培养液pH值是影响诱导效果的主要因素。Xylan as the inducing substrate, using ultrathin-layer agar plate diffusion technique and DNS assay and rescreening, eventually screened 3 xylanase producing high strains from 66 edible fungus in liquid fermentation induced culture. Black fungus LK8, Yutian mushroom, Mushroom 236 have better enzyme production capacity, detection of single factor combined with orthogonal experiment finalized training time, pH and xylan concentration as the optimal condition for xylanase production were 20 d, 7.5 and 1.0 g/100mL; 25 d,7.0 and 1.0 g/100mL; 25 d, 5.0 and 0.75 g/100mL, respectively. Xylanase activity under optimum conditions were： 600.6 IU/mL,672.5 IU/ mL,345 IU/mL. The culture solution pH value is the main factor affecting the induction effect .

关 键 词：木聚糖酶 食用真菌 诱导代谢 液体发酵 

分 类 号：S646[农业科学—蔬菜学]