脂质体包装农杆菌毒蛋白提高棉花转基因的效率  

Liposome Packaging Virulence Protein for Increasing the Efficiency of Transgenic Cotton

作　　者：刘红玲[1] 沈海涛[2] 祝建波[2] 邱红林[2] 王彦芹[3] 

机构地区：[1]石河子大学师范学院,新疆石河子832000 [2]石河子大学生命科学学院,新疆石河子832000 [3]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《江西农业大学学报》2014年第4期710-715,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31000730)

摘　　要：对比了表达载体pBin438-GFP、农杆菌毒蛋白与脂质体等6种不同的组合方式对棉花花粉通道法转基因效率的影响。结果表明脂质体包装显著提高了转基因棉花的成铃率,由16.5%提高到24.17%。对比不加脂质体所对应的组合都对转基因的效率也有明显的提高作用。在脂质体包装的不同组合中,农杆菌毒蛋白显著提高了转基因的效率,由1.4%提高到4.7%。本研究借助花粉管通道法转化的优势,导入T-DNA携带的目的基因GFP及脂质体包装的农杆菌毒蛋白(VirD1、VirD2、VirE2),提高了棉花基因转化效率,为棉花转基因育种提供更多的思路。The pollen tube pathway mothod is one of the methods for cotton transgenic technology. The effects of different combination modes of pBin438- GFP,virulence protein and liposome on the efficiency of transgenic cotton via pollen tube pathway. The result showed that the mode of liposome was beneficial to cotton boll rate,which increased from 16. 5% to 24. 17%. The mode of liposome added with virulence proteins（ VirD1,VirD2,VirE2） improved the transgenic efficiency significantly from 1.4% to 4.7%. Aiming at constructing a system for increasing the efficiency of transgenic cotton via pollen tube pathway,this study will brings up a new way for transgenic breeding techniques.

关 键 词：转基因棉花 花粉管通道法 T-DNA 脂质体 农杆菌毒蛋白 

分 类 号：S562[农业科学—作物学]