细胞相互作用和阿托伐他汀对内皮细胞与系膜细胞PAF及其受体基因表达的影响  

Effect of Atorvastatin on the Role of Platelet Activating Factor and Expression Level of Platelet Activating Factor Receptor in Mesangial Cells via Interaction of Endothelial Cells and Mesangial Cells

作　　者：周素娴[1] 李浩[1] 邓婷婷[1] 

机构地区：[1]桂林医学院附属医院内分泌科,桂林市545005

出　　处：《广西医学》2014年第10期1357-1359,共3页Guangxi Medical Journal

基　　金：国家自然科学基金资助项目(81260134);广西自然科学基金资助项目(2011GXNSFB018108);广西中医药管理局科研项目(GZKZ1146)

摘　　要：目的探讨高糖高脂条件下内皮细胞与系膜细胞相互作用对血小板活化因子(PAF)产生、系膜细胞血小板活化因子受体(PAF-R)基因表达的影响及阿托伐他汀的干预作用。方法内皮细胞与系膜细胞共培养和系膜细胞单独培养,分为对照组、甘露醇组、高糖+高溶血卵磷脂组、阿托伐他汀干预组,采用实时荧光定量检测系膜细胞PAF-R mRNA表达、酶联免疫吸附法检测PAF含量。结果共培养组和单培养组在高糖高溶血卵磷脂条件下,PAF和系膜细胞PAF-R mRNA表达均升高(P<0.05),单培养PAF均较共培养下降(P<0.05);阿托伐他汀可抑制高糖高溶血卵磷脂引起的PAF和系膜细胞PAF-R mRNA表达上调(P<0.05)。结论系膜细胞和内皮细胞在高糖高溶血卵磷脂培养下,存在相互作用,并促进PAF产生和系膜细胞PAF-R基因表达。阿托伐他汀可影响高糖高脂条件下内皮细胞与系膜细胞之间的相互作用,减少PAF的产生及下调系膜细胞PAF-R基因表达。Objective To investigate the role of platelet activating factor（PAF） and the expression of platelet activating factor receptor（ PAF-R） in mesangial cells affected by the interaction of endothelial cells and mesangial cells exposed to high glucose and lysophosphatidylcholine（LPC）,and to explore the effect of atorvastatin intervention.Methods Endothelial cells and mesangial cells were co-cultured while mesangial cells were cultured alone. The model of intercellular interaction between endothelial cells and mesangial cells was established and divided into 4 groups：control group,mannitol group,high glucose and LPC group,and atorvastatin group. The expression of PAF-R mRNA in mesangial cells was determined by quantitative real-time PCR. The content of PAF in mesangial cells was determined by enzyme-linked immunosorbent assay（ELISA）. Results The content of PAF and the expression level of PAF-R in mesangial cells increased when exposed to high glucose and LPC in co-culture and single-culture groups（P〈0.05）. The content of PAF of the monolayer cell culture was lower than that of the co-culture（P〈0.05）. Atorvastatin could inhibit the up-regulation of PAF and PAF-R mRNA expression in mesangial cells induced by high glucose and LPC（P〈0. 05）. Conclusion Exposed to high glucose and LPC,endothelial cells and mesangial cells can interact with each other to produce PAF. The expression of PAF-R in mesangial cells is promoted by high glucose and LPC. The intercellular interaction between endothelial cells and mesangial cells may be intervened by atorvastatin,atorvastatin can eliminate the excess of the PAF and down-regulate the expression of PAF-R in mesangial cells when exposed to high glucose and LPC.

关 键 词：系膜细胞 内皮细胞 阿托伐他汀 血小板活化因子 血小板活化因子受体 

分 类 号：R587.24[医药卫生—内分泌]