地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞凋亡的影响  

Effect of rehmannia glutinosa oligosaccharides on hydrogen peroxide combined serum deprivation-induced apoptosis in adipose tissue-derived stem cells

作　　者：裴志勇[1] 赵玉生[2] 高伟[2] 尹巧香[3] 

机构地区：[1]北京军区总医院干部病房一科,北京100700 [2]解放军总医院老年心血管病研究所 [3]空军总医院干部病房心内科

出　　处：《中国临床保健杂志》2014年第5期519-522,I0001,共5页Chinese Journal of Clinical Healthcare

基　　金：国家高科技研究发展计划(2006AA02A105)

摘　　要：目的探讨地黄低聚糖（RGOs）对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞（ASCs）凋亡的影响。方法过氧化氢（200μM）联合血清饥饿（H2O2/SD,6 h）建立小型猪ASCs凋亡模型。RGOs（0.01 g/L、0.1 g/L、1 g/L、10 g/L）预处理12h并继续干预6 h。Annexin V-FITC/PI检测细胞凋亡率,CCK-8法测定细胞活性,酶标仪比色法测定细胞caspase-3的活性。结果 RGOs在一定浓度范围（0.1-10 g/L）可以减轻H2O2/SD引起的ASCs损伤,表现在细胞凋亡率下降,细胞活性增加,caspase-3活性降低。结论地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞的凋亡具有保护作用。Objective To investigate the effect of Rehmannia glutinosa oligosaccharides（ RGOs） on hydrogen peroxide combined serum deprivation-induced apoptosis in adipose tissue-derived stem cells. Methods An apoptosis model of miniswine adipose tissue-derived stem cells（ ASCs） was established by hydrogen peroxide and serum deprivation（ H2O2/SD） in vitro. RGOs（ 0. 01 g /L,0. 1 g /L,1 g /L,10 g /L） were pretreated for 12 hours and incubated for 6 hours. Apoptosis of ASCs was determined by Annexin V-FITC /PI apoptosis kit,and the cell varibility and Caspase-3 activity was detected by CCK-8 assay and caspase-3 assay kit,respectively. Results RGOs significantly attenuated hydrogen peroxide combined serum deprivation-induced ASCs apoptosis,with a decreased apoptosis rate and caspase-3 activity,an increased cell varibility. Conclution RGOs has a protective effect on hydrogen peroxideand serum deprivation-induced apoptosis of adipose tissue-derived stem cells.

关 键 词：干细胞 细胞凋亡 地黄属 猪 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]