微乳液相色谱法测定丹参中隐丹参酮和丹参酮ⅡA的含量  

Simultaneous determination of cryptotanshinone and tanshinone ⅡA in Salvia miltiorrhizaby microemulsion liquid chromatography

作　　者：卢翠婷 冯晓华[1] 郭波红[1] 卫世杰[1] 李宁[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《西北药学杂志》2014年第5期441-443,共3页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81173525)

摘　　要：目的：建立丹参中隐丹参酮和丹参酮ⅡA的含量测定方法。方法采用微乳液相色谱法。色谱柱：DiamonsalC18（250mm×4．6mm，5μm）；流动相：0．14mol·L-1十二烷基硫酸钠-1．08mol·L-1正丁醇-0．070mol·L-1正辛烷-水微乳；流速：0．7mL·min-1；柱温：室温；检测波长：270nm。结果隐丹参酮和丹参酮ⅡA分别在0．962～38．48和1．022～40．88μg范围内线性关系良好，相关系数r＞0．9995；平均加样回收率（n＝6）分别为96．5％和97．2％，RSD分别为2．71％和1．69％。结论该方法准确，简单易行，可用于丹参药材中2种有效成分的含量分析。Objective To develop an MELC method for determining cryptotanshinone and tanshinone in Salviamiltiorrhiza simulta-neously .Methods A Diamonsil C18 column （250 mm×4 .6 mm ,5 μm） was used ,and the mobile phase consisted of 0 .14 mol · L-1 SDS-1 .08 mol · L -1 n-butanol-0 .070 mol · L -1 n-octane-water at a flow rate of 0 .7 mL · min-1 .The column temperature was room temperature ,and the detection wavelength was set at 270 nm .Results The linear range of cryptotanshinone and tanshinoneⅡA were within 0 .962-38 .48 and 1 .022-40 .88 μg ,respectively .The correlation coefficient r were 0 .999 5 and 0 .999 8 ,respec-tively .The average recoveries （n=6） were 96 .5% with RSD=2 .71% （n=6） and 97 .2% with RSD=1 .69% （n=6） .Conclu-sion This method was simple ,accurate and reliable ,and could be applied in the determination of the two components in Salvia miltiorrhiz a .

关 键 词：微乳液相色谱法 丹参 隐丹参酮 丹参酮ⅡA 

分 类 号：R284[医药卫生—中药学]