黄翘HPLC指纹图谱研究  

Study on the HPLC fingerprint of Fructus forsythiae

作　　者：崔誉文 杨黎彬[1] 杨静[1] 许苗苗[2] 程茜菲[2] 宋小妹[2] 崔九成[2] 

机构地区：[1]西安医学院,西安710021 [2]陕西中医学院,咸阳712046

出　　处：《西北药学杂志》2014年第5期446-449,共4页Northwest Pharmaceutical Journal

基　　金：陕西省中医管理局中医药科研课题(编号:ZY10)

摘　　要：目的建立黄翘HPLC指纹图谱,为黄翘的质量控制提供理论依据。方法色谱柱:Thermo C18Column(250mm×4.6mm,5μm);流动相:乙腈-水,梯度洗脱;检测波长:203nm。结果生成了黄翘的对照指纹图谱,标定了17个共有峰,并确定连翘苷、连翘酯苷A和表松脂素各峰的归属。结论黄翘的HPLC指纹图谱稳定,重复性良好,信息涵盖量大,为进一步科学评价黄翘的质量提供依据。Objective To establish an HPLC fingerprint of Fructus forsythiae .Methods The chromatographic separation was per-formed on a C18 column （250 mm × 4 .6 mm ,5μm） .The mobile phase was a mixture of acetonitrile-water solution in gradient elu-tion .The flow rate was 1 .0 mL · min-1 ,and the detection wavelength was 203 nm .Results The HPLC fingerprint of plantaginis semen showed 17 characteristic peaks with high similarity ,in which three peaks were identified .Conclusion The established method is simple ,accurate and reproduceable ,and can be used for the quality control of Fructus forsythiae samples .

关 键 词：黄翘 连翘苷 连翘酯苷A 表松脂素 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]