珠子参抗肝损伤药效的物质基础研究  

Preliminary study on the pharmacodynamic materials of Panax japonicus in anti-hepatic injury

作　　者：许苗苗[1] 张旋[2] 宋蓓[2] 王薇[1] 宋小妹[1] 

机构地区：[1]陕西中医学院,咸阳712046 [2]陕西中医学院第二附属医院,咸阳712000

出　　处：《西北药学杂志》2014年第5期486-489,共4页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81102805/H2804);陕西省科技统筹创新工程项目(编号:2011KTCQ03-02);陕西省教育厅专项科研计划项目(编号:2010JK490);陕西省教育厅省级重点实验室科研与建设计划项目(编号:09JS016)

摘　　要：目的探索珠子参抗肝损伤药效的物质基础。方法利用溶剂提取法、大孔树脂法及各种色谱技术,进行珠子参有效部位和化学成分的提取分离;采用CCl4所致小鼠化学性肝损伤模型,进行珠子参药效部位及有效成分筛选。将小鼠平均分为对照组、模型组、阳性对照组、珠子参不同剂量组,共9组。每天灌胃1次,连续7d。检测血清中AST和ALT含量的变化。结果珠子参总皂苷高剂量、中剂量可以降低ALT和AST含量,珠子参多糖低剂量组与模型组比较也可以降低ALT和AST的含量,而总皂苷高剂量有显著作用;竹节参皂苷Ⅳa各剂量组、人参皂苷Ro中、低剂量组均能降低ALT和AST的含量,与模型组对比,差异有统计学意义,竹节参皂苷Ⅳa低剂量效果显著。结论珠子参总皂苷为珠子参抗肝损伤的有效部位,主要化学成分竹节参皂苷Ⅳa为其抗肝损伤的主要有效成分。Objective To study the therapeutic material basis of Panax japonicus in anti-hepatic injury .Methods Chemical composi-tions of Panax japonicus were extracted and separated by macroporous resin column chromatography .The active ingredients were screened by using the mice model of acute liver injury .The mice were divided into nine groups ,normal control ,model group ,posi-tive control ,the total saponins and polysaccharide of Panax japonicus group with high ,medium and low-dose .The activities of ALT ,AST were examined after the administration of drugs orally for 7 days .Results Compared with the model control group ,the total saponins with high and medium dose and polysaccharide of Panax j aponicus group with low-dose could significantly decrease the level of ALT ,AST ;chikusetsusaponin Ⅳa all-dose group and ginsenoside Ro with medium and low dose group could signifi-cantly decrease the concentration of ALT ,AST .Conclusion The total saponins and chikusetsusaponin Ⅳa were effective parts and active ingredients for anti-hepatic injury .

关 键 词：珠子参 总皂苷 总多糖 竹节参皂苷Ⅳa 人参皂苷Ro 

分 类 号：R965[医药卫生—药理学]